Sequences from confirmation amplicon


Confirmation sequences for line 446E06 and hit At5g39970 are shown.
Line ID 446E06
Locus At5g39970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15999142
Primers used for PCR JH23 - 8474, show primer details
   
Sequence Name 65-K101386-0022-446-E06C-8409
GenBank Accession LN511357 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K101386-0022-446-E06C-8409
GCCTTTACATTGAATATATCCTGATCAAGAAACGGGTTAGTTTCATCTATTTTCAAAAGT
TCTCCTGATCCTTGAGCTGGAACTGTCACCAAGTATATCTTCCCGGGTTGATCAGACAGA
AAGACACGATTAGAACCATCAGGATGAGGTACCATGTTAAGATAAGACCCATTTCCAATC
TTCTCGATGCATATGCCTGAAGGAGATGATGGACCAGTAACTTTTGAAACGTTAAATGAA
ACTTCCTGACCATTGAAGCACACAGAGGATTCATCTGAAGCTCCACCAAAAATCTTGCAG
AAGTCATTACTTGATTTCCATATTTCAGATATTGTGGAAGTGATGTTGCCTCCATCTCCA
GCTTGAGATGCAAACGGAGTGTTGGTTACAGAGAGACTCTGGCATTCATTCCAAAATGTG
GCACAGAAATCAATGTCGGCTAGCGATTGTGTTGATTTACTAGAAGAAACTGTGGAGTTG
CAGAGCACAGGAACTTGTCTAGATTCTGACTCAACGCGGAAAAGCTCTGCTGCGAAAGGA
TCACACTTCTGCAACCACAGAAGGGAATCATCAACTGATTGCTAGAAAGGTTCTAAAATA
TCACGAACAAAACCACAGCATCCAAGTAATCTAATCACATTATGTTTTTGATATGTTTTT
GCCTTACCGAGCAAAGTAATGATTTGAGAAGTGGAGAACATCTGCCAGATACATTGAATG
CTTTAAACTGTCTCTGCAACTTCAAGTC
   
Sequence Name 65-K101392-0022-446-E06C-JH23
GenBank Accession LN511358 [GenBank]
Sequencing primer gene specific primer, JH23: 5'-TATGCTGTAACTCCCTGGAAGACT-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K101392-0022-446-E06C-JH23
TAAGCATTCATGTATCTGGCAGATGTTCTCCACTTCTCAAATCATTACTTTGCTCGGTAA
GGCAAAAACATATCAAAAACATAATGTGATTAGATTACTTGGATGCTGTGGTTTTGTTCG
TGATATTTTAGAACCTTTCTAGCAATCAGTTGATGATTCCCTTCTGTGGTTGCAGAAGTG
TGATCCTTTCGCAGCAGAGCTTTTCCGCGTTGAGTCAGAATCTAGACAAGTTCCTGTGCT
CTGCAACTCCACAGTTTCTTCTAGTAAATCAACACAATCGCTAGCCGACATTGATTTCTG
TGCCACATTTTGGAATGAATGCCAGAGTCTCTCTGTAACCAACACTCCGTTTGCATCTCA
AGCTGGAGATGGAGGCAACATCACTTCCACAATATCTGAAATATGGAAATCAAGTAATGA
CTTCTGCAAGATTTTTGGTGGAGCTTCAGATGAATCCTCTGTGTGCTTCANTGGTCAGGA
AGTTTCATTAACGTTTTCAAAAGTTACTGGTCCATCATCTCCTTCAGGCATATGCATCGA
GAAGATTGGAAATGGGTCTTATCTTAACAT

Last Updated on 06.12.2018 16:11