Sequences from confirmation amplicon


Confirmation sequences for line 448C09 and hit At1g64090 are shown.
Line ID 448C09
Locus At1g64090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23789749
Primers used for PCR BH72 - 8474, show primer details
   
Sequence Name 48-K104418-0022-448-C09C-BH72
GenBank Accession LN506500 [GenBank]
Sequencing primer gene specific primer, BH72: 5'-CAGAAACAAGTTTAGGGTTTTTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K104418-0022-448-C09C-BH72
GCGGAGAGCACAAGCACGAGGAATCGATCATGGAGAAGATCTCTGAGAAGATCCATGGTC
ACGATGACTCTTCTTCTTCTTCTTCCGATTCGGATGACGATAAGAACTCTGCGTCTCTCA
AAACCAAGATTTATCGTCTTTTCGGAAGAGAACAGCCTCTTCACAAGCTTTTCGGTGGCG
GAAAACGTATGGATCTCTCTCTCTTTTTCACCGATTTGCGATTTTAGTTACTATTTCTTA
TCGGATCTGGAATCTAGCCATTGATTGAGACTTAGGATTTGATTTGTTGCTTGGATTGAG
TATAGATTCAGAAAATCAGGATATAAACGAACGATTTGGTTTGGTGTTTGCTGGTTCTGG
AATCTGAGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGNTTATA
   
Sequence Name 64-K104419-0022-448-C09C-8409
GenBank Accession LN511197 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K104419-0022-448-C09C-8409
ATTCAGACCGCAAACCCAAACCAAATCGTTCGTTTATATCCTGATTTTCTGAATCTATAC
TCAATCCAAGCAACAAATCAAATCCTAAGTCTCAATCAATGGCTAGATTCCAGATCCGAT
AAGAAATAGTAACTAAAATCGCAAATCGGTGAAAAAGAGAGAGAGATCCATACGTTTTCC
GCCACCGAAAAGCTTGTGAAGAGGCTGTTCTCTTCCGAAAAGACGATAAATCTTGGTTTT
GAGAGACGCAGAGTTCTTATCGTCATCCGAATCGGAAGAAGAAGAAGAAGAGTCATCGTG
ACCATGGATCTTCTCAGAGATCTTCTCCATGATCGATTCCTCGTGCTTGTGCTCTTCCGC
CATTGTTAACTTTGAAACCTCCGCAAAAACCCTAAACTTGTTTCG

Last Updated on 06.12.2018 16:11