Sequences from confirmation amplicon


Confirmation sequences for line 449E05 and hit At5g10210 are shown.
Line ID 449E05
Locus At5g10210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3206481
Primers used for PCR JH19 - 8474, show primer details
   
Sequence Name 69-K101328-0022-449-E05C-8409
GenBank Accession LN512158 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K101328-0022-449-E05C-8409
TTTACAATTGAATATATCTTCAATTCCTCGACCGCGGTTTCGTTCCCTTGGCACTCCAGA
CCGAATGATTGGTTCCAGGAAATGACCTCACAGTCTGATCTCGAACGGATCTCTGGAGTG
TTTAGTTCAATCTTACTGTTATTCCCTGCATGGAGATAGAACCTAACGAAGAGAGTGGTT
GGCGATGACTTGAGCTCTACGTTTCTAGCTTCCACGATTTTCACTTCAAATTTAAGGGAA
CGAATGTTTTCAACGATACCCATTTGCTTGAAAATTTCGAATCGAACCAAGAATATATTA
TTTACCAAGGTAATAACAATTAGAGCAAATTTAAGAGCAGTGAGTTCCTTTGTGA
   
Sequence Name 69-K101334-0022-449-E05C-JH19
GenBank Accession LN512159 [GenBank]
Sequencing primer gene specific primer, JH19: 5'-TCACAAAGGAACTCACTGCTCTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K101334-0022-449-E05C-JH19
TATTCTTGGTTCGATTCGAAATTTTCAGCAAATGGGTATCGTTGAAAACATTCGTTCCCT
TAAATTTGAAGTGAAAATCGTGGAAGCTAGAAACGTAGAGCTCAAGTCATCGCCAACCAC
TCTCTTCGTTAGGTTCTATCTCCATGCAGGGAATAACAGTAAGATTGAACTAAACACTCC
AGAGATCCGTTCGAGATCAGACTGTGAGGTCATTTCCTGGAACCAATCATTCGGTCTGGA
GTGCCAAGGGAACGAAACCGCGGTCGAGGAATTGAAGATATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT

Last Updated on 06.12.2018 16:11