Sequences from confirmation amplicon


Confirmation sequences for line 449E09 and hit At3g16570 are shown.
Line ID 449E09
Locus At3g16570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5645141
Primers used for PCR 06cc - 8474, show primer details
   
Sequence Name 29-K108223-0022-449-E09C-06cc
GenBank Accession LN499321 [GenBank]
Sequencing primer gene specific primer, 06cc: 5'-AACCAAATCCTATTATCGCCTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K108223-0022-449-E09C-06cc
ACTTTGGTTTGACGAATTATTATTTTATTTAGTTAGTGATCATACTTCTTCATATGGAAA
CTTATAATTATAGTTTATGAATTAGAAAACCTTATTTTATTTTTCTAGCATCTGGTATTT
CTTCGGCACATGCATGCATGATGCATCCATGGACCATAAATACGAAAACAAATCCATTTC
ATATATATTTACTTCAATAACAAATTCGTTTATGAGTGATGATACTATTAATTATAATGT
ATTTATTCCATAGTTTTTTTTCTGGTTTATGTAAACAAACTTACATCAATAACAGAGATA
AATTCCCAGAAACATTTACATGTGTATGAAACTCTAATAATCCGCAATGAACTTAGAAAT
ATAAACCGGTAACAAAAGAAAAGAGAAATTTCTGAAAAATTATATATCAGAAAAAGAAAA
GAAAAAAGAATTACAGTGAGACTGACAGTGATAGATTCATGAGCGCCGGCAGCGAGTGAT
AGACTTACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCACGNTTATT
   
Sequence Name 13-K108222-0022-449-E09C-8409
GenBank Accession LN492072 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K108222-0022-449-E09C-8409
GCCTTTACAATTGAATTATCCTGTAAGTCTATCACTCGCTGCCGGCGCTCATGAATCTAT
CACTGTCAGTCTCACTGTAATTCTTTTTTCTTTTCTTTTTCTGATATATAATTTTTCAGA
AATTTCTCTTTTCTTTTGTTACCGGTTTATATTTCTAAGTTCATTGCGGATTATTAGAGT
TTCATACACATGTAAATGTTTCTGGGAATTTATCTCTGTTATTGATGTAAGTTTGTTTAC
ATAAACCAGAAAAAAAACTATGGAATAAATACATTATAATTAATAGTATCATCACTCATA
AACGAATTTGTTATTGAAGTAAATATATATGAAATGGATTTGTTTTCGTATTTATGGTCC
ATGGATGCATCATGCATGCATGTGCCGAAGAAATACCAGATGCTAGAAAAATAAAATAAG
GTTTTCTAATTCATAAACTATAATTATAAGTTTCCATATGAAGAAGTATGATCACTAACT
AAATAAAATAATAATTTCGTACAAACCAAAGTTGTATATTGAGGGCGAATAAAGAGGCGA
TAATAGGATTTGGT

Last Updated on 06.12.2018 16:11