Sequences from confirmation amplicon


Confirmation sequences for line 449G02 and hit At3g50240 are shown.
Line ID 449G02
Locus At3g50240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18628145
Primers used for PCR 20dr - 8474, show primer details
   
Sequence Name 56-K109780-0022-449-G02C-20dr
GenBank Accession LN509129 [GenBank]
Sequencing primer gene specific primer, 20dr: 5'-GCTTCTTATGGAGCATGGGTATAG-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K109780-0022-449-G02C-20dr
TTGGTGCTCCAGTTGCCCTGGTTAGAGATCCTTGCTCTAGGCAGGAAGCCATTTCTTCTT
TAGTAGCTATGGGAACTTCAGTTGCACCGGATAGTGTTATAACCCCGTTTGGTGATTCAC
GAATCTGCACCGGTGACTTACTAAGAACAACCTTCCCAGGGGTTCCATTGGCTAGTCTGT
TAAACGGCACACTACTATCCAGCAAATCCAGAACTTCTTCTTTTAAAATCTACAGATTAA
GATTTCAGTTAATATCCATACAACTATAAATCTAGACAAATTAATCTAGCAACCAACCTC
GATGAAAGAGACATGTAACTGGAACCCCATTTGGTGCTTTACTGAATCAATTTTGTTGAA
CAAAGCACTCATAACTTGAGGAATCAGCCCATTTTTTGTACCATCTTTAATACCAGTCCC
CATAGTGTATGTCTTTCCAGATCCTGTCTGCATCATTTATGTAACTCAAATAGAAGAAAA
CACAAGCAGCTAAAACCAAAAATGGCAAAGAGGAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCACAACGT
TATTA
   
Sequence Name 40-K109779-0022-449-G02C-8409
GenBank Accession LN503735 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K109779-0022-449-G02C-8409
TTTTCTTCTATTTGAGTTACATAAATGATGCAGACAGGATCTGGACAGACATACACTATG
GGGACTGGTATTAAAGATGGTACAAAAAATGGGCTGATTCCTCAAGTTATGAGTGCTTTG
TTCAACAAAATTGATTCAGTAAAGCACCAAATGGGGTTCCAGTTACATGTCTCTTTCATC
GAGGTTGGTTGCTAGATTAATTTGTCTAGATTTATAGTTGTATGGATATTAACTGAAATC
TTAATCTGTAGATTTTAAAAGAAGAAGTTCTGGATTTGCTGGATAGTAGTGTGCCGTTTA
ACAGACTAGCCAATGGAACCCCTGGGAAGGTTGTTCTTAGTAAGTCACCGGTGCAGATTC
GTGAATCACCAAACGGGGTTATAACACTATCCGGTGCAACTGAAGTTCCCATAGCTACTA
AAGAAGAAATGGCTTCCTGCCTAGAGCAAGGATCTCTAACCAGGGCAACTGGGAGCACCA
ATATGAATAATGAGTCGAGGTGTGTATCTCTATACCCATGCTCCATA

Last Updated on 06.12.2018 16:11