Sequences from confirmation amplicon


Confirmation sequences for line 453F12 and hit At4g38920 are shown.
Line ID 453F12
Locus At4g38920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:18147358
Primers used for PCR JH16 - 8474, show primer details
   
Sequence Name 68-K101386-0022-453-F12C-8409
GenBank Accession LN511970 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K101386-0022-453-F12C-8409
CCATTTCTTTTTCTCCTTTCTTCGGCTTCCTTGGCGCTGCCGCTGCGCTCGTCTTCTCAT
GTATATCCATCTATACTCTCTTATAATCTCCATCTTCTTCTTTTCTCTTTGGTTCTGAAT
TGTTCACTGATCTTGTGTGTGTTTGTTGTTCTCATGATCTCCCTCAAAGTTGCTTTTGTG
CTTTAGATCGGCCCTGAGAAGTTCACCGATCTGTGTGTTGCTCATGATTTTTCTTAGAAA
TTTCTCTCTAGATTTGTTTAGGCTAAGCGATATCACTGAACTATTATATGTCTCTCCTTT
TCGAAAATTCTAGTTTATTATCTTTAGTCAGTGGATCAAATTCTTATGGAACTGCTCTAC
GCTGGTTTTAAATCCTTTTTCTTTGTCTTTTCTCCATTGGTGATGTAGT
   
Sequence Name 68-K101392-0022-453-F12C-JH16
GenBank Accession LN511971 [GenBank]
Sequencing primer gene specific primer, JH16: 5'-TCAGCCTAGACTACATCACCAATG-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K101392-0022-453-F12C-JH16
TAAACCGCGTAGAGCAGTTCCATAAGAATTTGATCCACTGACTAAAGATAATAAACTAGA
ATTTTCGAAAAGGAGAGACATATAATAGTTCAGTGATATCGCTTAGCCTAAACAAATCTA
GAGAGAAATTTCTAAGAAAAATCATGAGCAACACACAGATCGGTGAACTTCTCAGGGCCG
ATCTAAAGCACAAAAGCAACTTTGAGGGAGATCATGAGAACAACAAACACACACAAGATC
AGTGAACAATTCAGAACCAAAGAGAAAAGAAGAAGATGGAGATTATAAGAGAGTATAGAT
GGATATACATGAGAAGACGAGCGCAGCGGCNGCGCCNAGGAAGCCGAAGAAAGGAGAAAA
AGAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC

Last Updated on 06.12.2018 16:11