Sequences from confirmation amplicon


Confirmation sequences for line 453G07 and hit At1g13440 are shown.
Line ID 453G07
Locus At1g13440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4610218
Primers used for PCR HM67 - 8474, show primer details
   
Sequence Name 48-K101219-0022-453-G07C-8409
GenBank Accession LN506452 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K101219-0022-453-G07C-8409
TTACATTGAATAATCCTGCTCGTCGCTGTTAACGATCCTTTCATCACCACCGAGTACATG
GTTCGTTTCTTCTTCTCTTCGATACTAGTTCGTATCTGGATTTGAGTTAATAGTTTTATC
TGAATTTTTGTTAATATACATGATGGTGTGTGCTGTTTATCATATAGATCTGAGAACTTG
CTTTGTTGTATTCTTCTTCTATGTTGATTCTCTGTAAATTCGTTTTGACTGATTGTGTAT
ATGGTATCGATTTGTTGCAGACATACATGTTTAAGTATGACAGTGTTCACGGTCAGTGGA
AGCACCATGAGCTTAAGGTGAAGGATGACAAAACTCTTCTCTTCGGTGAGAAGCCAGTCA
CTGTTTTCGGCATCAGGTACATAATCATGGATGATTTTATTATAGTGGTTCTCTGTTATG
TTATTGAGAGTTGTGAAATTCTGATTTGTGGTGTTTTATTTTGAAAGGAAC
   
Sequence Name 48-K101226-0022-453-G07C-HM67
GenBank Accession LN506453 [GenBank]
Sequencing primer gene specific primer, HM67: 5'-GGTTCCTTCAAAAATAAAACACCA-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K101226-0022-453-G07C-HM67
ATCCACTCTCATAACTACAGAGAACACTATATAAAATCATCCATGATTATGTACCTGATG
CCGAAAACAGTGACTGGCTTCTCACCGAAGAGAAGAGTTTTGTCATCCTTCACCTTAAGC
TCATGGTGCTTCCACTGACCGTGAACACTGTCATACTTAAACATGTATGTCTGCAACAAA
TCGATACCATATACACAATCAGTCAAAACGAATTTACAGAGAATCAACATAGAAGAAGAA
TACAACAAAGCAAGTTCTCAGATCTATATGATAAACAGCACACACCATCATGTATATTAA
CAAAAATTCAGATAAAACTATTAACTCAAATCCAGATACGAACTAGTATCGAAGAGAAGA
AGAAACGAACCATGTACTCGGTGGTGATGAAAGGATCGTTAACAGCGACGAGCAGGATAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTC

Last Updated on 06.12.2018 16:11