Sequences from confirmation amplicon


Confirmation sequences for line 454E07 and hit At5g57710 are shown.
Line ID 454E07
Locus At5g57710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23386222
Primers used for PCR 24uj - 8474, show primer details
   
Sequence Name 44-K117932-0022-454-E07C-24uj
GenBank Accession LN505326 [GenBank]
Sequencing primer gene specific primer, 24uj: 5'-GGACTCATAGGCTTCAAGTGTACC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K117932-0022-454-E07C-24uj
GCTGCACGGCTGACGAAAAGCATGTTAGGACTGTAAGGGCTCGTTGTCAACGTTATAGGA
ACCGGGATCGGAACAATCCTCTCGTTCTTGTTATGAAAGCTAGGATGAAGACGCACGCAT
GCATCATTCCATTTCTTCTGCACTTCTTCAATCTTTGCTTGCTGTAATAAAAACATTACA
AAACTGATAAATTTATGATCTGCAATCATATCATAAAGCCATTGCAATTGTTGAAGAGAA
GATTCAATAACCGGAACTTACCGGTAAACGATCAACCGGTTTAGCTTTCAACAGCCACTG
TGGCAACTGTTTAGGCAATGAGTTGAAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTACATGTCCGCACGAATTATTAT
   
Sequence Name 04-K117931-0022-454-E07C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K117931-0022-454-E07C-8409
TTGCCTTTGTTGAAGAGAAGATTCGGTATCCGGAACTTACCGGTCAACGATCAACCGGTT
TAACTCTCAACAGCCACTGTGGCCACCGTCTAGGCCATGAGTTGTAAATGGCTCCATGTC
CGGGAAATCTGCGTGGATCGGCACTGATTATGATGGTCAATCCAGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT

Last Updated on 06.12.2018 16:11