Sequences from confirmation amplicon


Confirmation sequences for line 455G05 and hit At5g14550 are shown.
Line ID 455G05
Locus At5g14550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4692739
Primers used for PCR 17nt - 8474, show primer details
   
Sequence Name 52-K114572-0022-455-G05C-17nt
GenBank Accession LN507894 [GenBank]
Sequencing primer gene specific primer, 17nt: 5'-ACCGCTTTGTTTTTCTTTCTGATA-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K114572-0022-455-G05C-17nt
TTTGGATTGTAGTTGATGACTTGCTAACGAATTTTTTAGTTTTTTTGGTTTCTTCTCAGC
TGCATACCTCTGTACAGTTTCAGCTACACGTATAACTACATCATGTCAACACCAACTAGT
TTCGTTGATAGGTCCGTGCTGCTTATTTACATAACTCCATATTTCAACTTTGACACCAAA
TTACTAAATATTTTTCTGTATTGCAGCTTTGCAGATACAAAAGATAGCCGTTATAATCCT
AGAATGAATCCCATTATTCCTGTTCGTAACTGGAGAAAAGGATCACAGGTGAAGTTCCAA
ATCACTTGTAAATATGAAATCTACATGTCCGGATATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCAC
   
Sequence Name 40-K114571-0022-455-G05C-8409
GenBank Accession LN503795 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K114571-0022-455-G05C-8409
TTGAATATATCCGGACATGTAGATTTCATATTTACAAGTGATTTGGAACTTCACCTGTGA
TCCTTTTCTCCAGTTACGAACAGGAATAATGGGATTCATTCTAGGATTATAACGGCTATC
TTTTGTATCTGCAAAGCTGCAATACAGAAAAATATTTAGTAATTTGGTGTCAAAGTTGAA
ATATGGAGTTATGTAAATAAGCAGCACGGACCTATCAACGAAACTAGTTGGTGTTGACAT
GATGTAGTTATACGTGTAGCTGAAACTGTACAGAGGTATGCAGCTGAGAAGAAACCAAAA
AAACTAAAAAATTCGTTAAGCAAGTCATCAACTACAATCCAAACATGAAAAGGTATTAAC
AGAATCAATCTCACCTATNAAAAAGAAAACAAAAGCGG

Last Updated on 06.12.2018 16:11