Sequences from confirmation amplicon


Confirmation sequences for line 455H07 and hit At4g10660 are shown.
Line ID 455H07
Locus At4g10660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6583453
Primers used for PCR 16ww - 8474, show primer details
   
Sequence Name 41-K118874-0022-455-H07C-16ww
GenBank Accession LN504268 [GenBank]
Sequencing primer gene specific primer, 16ww: 5'-CATGCTCTCTTAGATTTGACCCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K118874-0022-455-H07C-16ww
ACGATCACTTCTATTGTTTAGCTCAGGAGGTGTTTTAGTTGACTTTGATGTAGCTTCCCG
CACAATGAGTGAACAAGCATGGAATGAACATAGATGCGCTTATATGCGTAATGATAATGC
AGAATGGTTTAATCTTCCGAAGAGAATTTACTTGGCTGAGCAGAAAGGAGAGCTCTTTCT
CATGTATACATGTAGCAGCGAGATACCGATGGTGTATAAACTTGTCTCCTCAAATTGGGA
GGAGATAAATAGTACTACTCTTGACGGCGTGACAATCTTTGCAAGTATGTATTCCTCGGA
GACGAGATTGGATGTTCTTGGGATGAGGAACAGTGTGTACTTCCCAAAGTATGGTTTAGA
TTGCAAGGGATGTGTATCCTACTCGTTTGATGAAGCCAGGTACTATCCGCGTAAGCAGCT
TCCAAAACCAACAATATGGCAAATGCAGAAAGAACTTTAAATTGCATCCATATTTGATGC
AGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTC
   
Sequence Name 01-K118873-0022-455-H07C-8409
GenBank Accession LN486796 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K118873-0022-455-H07C-8409
ATTGAATATATCCTGCATCAATATGGATGCAATTTAAAGTTCTTTCTGCATTTGCCATAT
TGTTGGTTTTGGAAGCTGCTTACGCGGATAGTACCTGGCTTCATCAAACGAGTAGGATAC
ACATCCCTTGCAATCTAAACCATACTTTGGGAAGTACACACTGTTCCTCATCCCAAGAAC
ATCCAATCTCGTCTCCGAGGAATACATACTTGCAAAGATTGTCACGCCGTCAAGAGTAGT
ACTATTTATCTCCTCCCAATTTGAGGAGACAAGTTTATACACCATCGGTATCTCGCTGCT
ACATGTATACATGAGAAAGAGCTCTCCTTTCTGCTCAGCCAAGTAAATTCTCTTCGGAAG
ATTAAACCATTCTGCATTATCATTACGCATATAAGCGCATCTATGTTCATTCCATGCTTG
TTCACTCATTGTGCGGGAAGCTACATCAAAGTCAACTAAAACACCTCCTGAGCTAAAACA
ATAGAAGTGATCGTTGGCATAGACAAGGTTGCTATGCATGTATGGGTCAAATCTAGAA

Last Updated on 06.12.2018 16:11