Sequences from confirmation amplicon


Confirmation sequences for line 456H02 and hit At3g19830 are shown.
Line ID 456H02
Locus At3g19830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6888758
Primers used for PCR 25uj - 8474, show primer details
   
Sequence Name 25-K117356-0022-456-H02C-25uj
GenBank Accession LN497631 [GenBank]
Sequencing primer gene specific primer, 25uj: 5'-GAAGTTTCTGGGCATTTACAAGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K117356-0022-456-H02C-25uj
ATCTTGTTCCTTCCGCATTTCTCCGATTTAAGTCTTCTGAACAGGCCCAACAGCTTTTCC
CTTCTGGAAATCCAAGACAATTTTCTTTGGGCGTACAAATAATCGAGGCAAATCTTCTGT
CAGTAGTTTGGTCAAGAACCTGAAAGAGATGAAGCAAAGGAAGGAGGAAAGAAACTGATT
TATCAATACGTCGGAAGAGAGTAAACAATCAACAGAGAGAAGAAGCTAAAACGAATACAT
TGAGGAGACTTACATTGATAAAACAGGAATTCCTGATGTTGTCATCCAAAGCATTCATGC
AAAAAGAAGAAAATGATTAAAGCTATTACTAATGTACATGATTATAAAAATCAAAGGAGA
TAATGAGAAAGCAACAGACAATTATGATTAACTAATACCCATTAGATTAAACAATCGGAA
TGGTGCAAGCTCAAATTTGATCTTTGGAAGTGAAACAAACGCCCATGATGCTGCTCCAAC
CCAAGGCGCTGACGGAATCAATCTTAATTTAGATGGATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 05-K117355-0022-456-H02C-8409
GenBank Accession LN488527 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K117355-0022-456-H02C-8409
TTGAATATCCATCTAAATTAAGATTGATTCCGTCAGCGCCTTGGGTTGGAGCAGCATCAN
TGGGCGTTTGTTTCACTTCCAAAGATCAAATTTGAGCTTGCACCATTCCGATTGTTTAAT
CTAATGGGTATTAGTTAATCATAATTGTCTGTTGCTTTCTCATTATCTCCTTTGATTTTT
ATAATCATGTACATTAGTAATAGCTTTAATCATTTTCTTCTTTTTGCATGAATGCTTTGG
ATGACAACATCAGGAATTCCTGTTTTATCAATGTAAGTCTCCTCAATGTATTCGTTTTAG
CTTCTTCTCTCTGTTGATTGTTTACTCTCTTCCGACGTATTGATAAATCAGTTTCTTTCC
TCCTTCCTTTGCTTCATCTCTTTCAGGTTCTTGACCAAACTACTGACAGAAGATTTGCCT
CGATTATTTGTACGCCCAAAGAAAATTGTCTTGGATTTCCAGAAGGGAAAAGCTGTTGGG
CCTGTTTCAGAAGACTTAAAATCTGGAGAAATGCAGGAAGGGAACAAGGATTTTGTAGGG
GAACTGTCTGTTACTCTTGTAAATGCCCGGAAAACTTC

Last Updated on 06.12.2018 16:11