Sequences from confirmation amplicon


Confirmation sequences for line 458B09 and hit At4g34380 are shown.
Line ID 458B09
Locus At4g34380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16439839
Primers used for PCR 25qz - 8474, show primer details
   
Sequence Name 22-K116405-0022-458-B09C-25qz
GenBank Accession LN496268 [GenBank]
Sequencing primer gene specific primer, 25qz: 5'-TTCCGCTGTATATAATCCACTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K116405-0022-458-B09C-25qz
GCCCGTTCCTCCTCACGGCTAGGCACTTGACCGGCCCCATGTGTCCCGTCAAAACAGAGA
GACACTGATGGGACTTGTCAGACGGGTCCCGCCTCCATACGCATATGTTCTTGTCGGCGG
AACCACTCAACAGCAAGTTCCCCGCTATACCTAGACAAAGCACTGCGGATTTGTGGCCTT
TGAGTATTCCGCCTGTGAATGACCGCTTTGAACGCTCCCAATAGTTCACTAGCCCGTCCG
AAGAACCGCAATATACGATGGAAGATTGTGATTTTACGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCGCACAGATATAATAAT
   
Sequence Name 12-K116404-0022-458-B09C-8409
GenBank Accession LN491742 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K116404-0022-458-B09C-8409
GTAATCCATCTTCCTCGTATATTGCGGTTCTTCGGACGGGCTAGTGAACTATTGGGAGCG
TTCAAAGCGGTCATTCACAGGCGGAATACTCAAAGGCCACAAATCCGCAGTGCTTTGTCT
AGGTATAGCGGGGAACTTGCTGTTGAGTGGTTCCGCCGACAAGAACATATGCGTATGGAG
GCGGGACCCGTCTGACAAGTCCCATCAGTGTCTCTCTGTTTTGACGGGACACATGGGGCC
GGTCAAGTGCCTAGCCGTGGAGGAGGAACGGGCTTGTCATCAGGGAGCAAAAGCCTCAGT
GGCCGAGGGAGACCGGAAGTGGATTATTANCAGCGGAAA

Last Updated on 06.12.2018 16:11