Sequences from confirmation amplicon


Confirmation sequences for line 458H02 and hit At2g17720 are shown.
Line ID 458H02
Locus At2g17720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7698916
Primers used for PCR 91gp - 8474, show primer details
   
Sequence Name 40-K112586-0022-458-H02C-91gp
GenBank Accession LN503758 [GenBank]
Sequencing primer gene specific primer, 91gp: 5'-TTTGGAAATCCCTTATTGAGTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K112586-0022-458-H02C-91gp
TCTTCAGTTCTTCACTACCAAGTTGGGCAGAAGTATGAGCCTCACTATGACTATTTCTTA
GATGAGTTCAACACCAAGAATGGAGGACAACGAATAGCTACTGTGCTTATGTACCTGTAA
GTAACTCACAAAACTCATTATTCCTTTTTCTCAGAGCATTTCTGTGTTTAATCTTTCTTT
TTTTTTCACACGATTCTTTAGCTCTGATGTCGATGATGGTGGCGAGACTGTGTTCCCTGC
AGCAAGAGGAAACATTAGTGCTGTCCCATGGTGGAACGAGCTCTCAAAATGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 14-K112585-0022-458-H02C-8409
GenBank Accession LN492576 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K112585-0022-458-H02C-8409
CATTTCATTTTGAGAGCTCGTTCCACCATGGGACAGCACTAATGTTTCCTCTTGCTGCAG
GGAACACAGTCTCGCCACCATCATCGACATCAGAGCTAAAGAATCGTGTGAAAAAAAAAG
AAAGATTAAACACAGAAATGCTCTGAGAAAAAGGAATAATGAGTTTTGTGAGTTACTTAC
AGGTACATAAGCACAGTAGCTATTCGTTGTCCTCCATTCTTGGTGTTGAACTCATCTAAG
AAATAGTCATAGTGAGGCTCATACTTCTGCCCAACTTGGTAGTGAAGAACTTGAAGACCT
TCACCATTTTCTACAAACAAAAGCGTCATCCACAAACTCAATAAGGGATTTCCAA

Last Updated on 06.12.2018 16:11