SimpleSearch - Line and FST details


Line specific information

 
Line ID 458H02
Vector Used pAC161
Line Availability available as T3 set from NASC (N443958)
Segregation Analysis N/A (line seemingly not resistant, T2 plants grown without drug)
Confirmed for Hit At2g17720
Parent of DUPLO pair none
Parent of pair(s) 3036, 3070, 96768

Gene hit At3g05660

 
Sequence (A. th genome BLAST matches underlined) 
>16-K018858-022-457-H02-8409
ACGAGTTTACGATTAAGAACCTTGTTTTGGTTGTCCAGTCCTCTGAAGACAAAGTCATGG
GAGAATGGCAGCGACTGTTGTCATTGGGATGGTATTACTTGCGATGCTAAGACCGGGGAA
GTAATCGAGATAGACCTTATGTGCAGCTGCCTCCATGGCTGGTTTCATTCCAACAGTAAT
CTTTCTATGCTTCAAAATTTCCATTTTCTAACCACCCTATGGGATCCTCCCCTATAGTGA
GANNNNNNNNNNNNNNNNNNNNNCCNNNTTTTTNTNNNNTNNNNAANGNNNNNGCNNNNN
NGNTNNNTNATAANNNTNNNNANNTNNTNNTTTTTTTATAAANTAANTATAATTTNAAAT
ATATTAAAAAATTATTTTAAAATATAATTATTATTGAAATANAAATAATTTTNAAAATAT
TAATTTATGATGAGAAGATATATTTGGNGTTGGGTNTANTGAGTAGGATAGTTGGAGAAT
GGTATATTAGAAAAAAAATGAGGGGTAGATGAGGATGATTAGTATTTTTTTGTATGGATA
GGTGTGAAATGGAAATTATGTAAGAAATGTTCGTGAAATATTGGAGGGAGGGTTGGGATG
GTNGTGTATTTGAAAATGTGAATTGA
GenBank Accession BX292901 [GenBank]
Graphic View Graphic view of gene At3g05660
Predicted Position of Insertion Chr3:1651874 - go to primer design
BLAST e Value 2e-107
Hit Clone Code (BAC ID) F18C1
Hit Gene Code At3g05660 [Araport] [TAIR] [MIPS] [SIGnAL]
Gene Annotation receptor like protein 33
Insertion Classification CDSi
Confirmation Status unknown

Gene hit At2g17720

 
Sequence (A. th genome BLAST matches underlined) 
>16-K018852-022-457-H02-8409
CATTGTGAGAGCTCTCTCCACACTGGGACAGCACTAATGGTTCCTCATTGCTGCACGTAA
CAATGGCTCTCCTCCATCATCTACATCAGAGCTAAAGAATCGTGTGAACCTAATAGACAG
ATTGCCCCCATGGATGCTCTCATACCAAGGAATATCGATCTATGCGACTTACTTACAGGT
ACCTAACCACACTATCTAATCCTGGTCCTCCATTCTTGGTGTTGAACTCATCTAAGAAAT
AGTCATAGTGAGGCTCATACTTCTGCCCAACTTGGTAGTGAAGAACTTGAAGACCTTCAC
CATTTTCTACAAACAAAAGCGTCATCCACAAACTCAATAAGGGATTTCCAAAGGAACACA
ACTCAAGATATCAAGAGTAAATGCAACTGATGACCTCAAGATGTGTCAACATTTCCTGCT
GTGCATCTAAGTATTGGATAACAAAGCAAAGAGCACTTACCAACAGGAATGAAGGTGAAA
TCTGAAATCCTTTTCTCAATCACCTCGACAACTTCGTCATGTCCTCTTCTAAGAAAAGTC
CTGAGCTAT
GenBank Accession BX292900 [GenBank]
Graphic View Graphic view of gene At2g17720
Predicted Position of Insertion Chr2:7698916 - go to primer design
BLAST e Value 0.0
Hit Clone Code (BAC ID) T17A5
Hit Gene Code At2g17720 [Araport] [TAIR] [MIPS] [SIGnAL]
Gene Annotation 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Insertion Classification CDSi
Confirmation Status confirmed, show confirmation sequences
Primer and wt-amplicons show primer details


Last Updated on 10.06.2021 13:37