Sequences from confirmation amplicon


Confirmation sequences for line 459C01 and hit At5g49830 are shown.
Line ID 459C01
Locus At5g49830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20253683
Primers used for PCR KF22 - 8474, show primer details
   
Sequence Name 55-K103224-0022-459-C01C-KF22
GenBank Accession LN508777 [GenBank]
Sequencing primer gene specific primer, KF22: 5'-TGAGAGAGCTGAGGGAAAAGTTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K103224-0022-459-C01C-KF22
AAAGAGTGTGTTTGATTTGGTTAACTAGATTGTTGGATTACTTGGCTGGACTCTTTGTTT
TTTCTCAGTTATTTTGGGATGATGACATATTTCTGTTCGGTTCACAGGATATAAAGCAGT
TATGCTCGTATCTATTGGATTTGAAGAGAGCATCTGCTGAAGAAATGCGGCGAAGTGTTT
ATGCTAATTATCCAGCCTTTATACGGTTAGCTGATATCCTGTCTGTTTTTAGTGCTTGGT
TATTTTCTTTGAAGTTGATGTGATAATAAAGCTCTACTTTTTCAAGTTATTTCATTTCTT
GTGGGTATTGGTGCATTTGCGTTTTGCATATGACTTCTTTTTCATTTCTTTTTCCTTGCA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCACGATTATA
   
Sequence Name 23-K103225-0022-459-C01C-8409
GenBank Accession LN496514 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K103225-0022-459-C01C-8409
CCATTTACATTGAATATGCAAGGAAAAAGAAATGAAAAAGAAGTCATATGCAAAACGCAA
ATGCACCAATACCCACAAGAAATGAAATAACTTGAAAAAGTAGAGCTTTATTATCACATC
AACTTCAAAGAAAATAACCAAGCACTAAAAACAGACAGGATATCAGCTAACCGTATAAAG
GCTGGATAATTAGCATAAACACTTCGCCGCATTTCTTCAGCAGATGCTCTCTTCAAATCC
AATAGATACGAGCATAACTGCTTTATATCCTGTGAACCGAACAGAAATATGTCATCATCC
CAAAATAACTGAGAAAAAACAAAGAGTCCAGCCAAGTAATCCAACAATCTATGTTAACCA
AATCAAACACTACTCTTTTTGTAGTAAACTCTAGAAGGCCTAAACTAACTTTTCCCTCAG
CTCTAT

Last Updated on 06.12.2018 16:11