Genotyping and primer details


Genotyping and primer details for 459C01 - At5g49830
Line ID 459C01
Locus At5g49830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:20253683 of line 459C01 Chr5:20253683
   
Insertion allele amplicon 8474 - KF22
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KF22: 5'-TGAGAGAGCTGAGGGAAAAGTTAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:20254077
   
wt amplicon (over InsPos) 23sq - KF22
wt amplicon start Chr5:20253298
wt amplicon end Chr5:20254077
wt amplicon size 780 bp
Locus specific primer KF22: 5'-TGAGAGAGCTGAGGGAAAAGTTAG-3'
2nd locus specific primer 23sq: 5'-GCTTAAAGTATGCTTTTCGTTTGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11