Sequences from confirmation amplicon


Confirmation sequences for line 459G12 and hit At3g62750 are shown.
Line ID 459G12
Locus At3g62750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:23214515
Primers used for PCR 58sj - 8474, show primer details
   
Sequence Name 54-K116453-0022-459-G12C-58sj
GenBank Accession LN508595 [GenBank]
Sequencing primer gene specific primer, 58sj: 5'-ATGAATTGGCTTTAGTTCCGT-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K116453-0022-459-G12C-58sj
CTCTTTGATGAGATTCTTGTAAACAATAAACCTTTTGGGTTAATGCGTCCTCTTCCATCT
GAAAAATGACACAGACAAAAAGGGTCTAATATAAGAGGTCAGAGACAACATTATTAGTTA
CTTGGGAATTAAGAAACCAAGAATAGGCGTTACTAGGTATAAGTCTTGACCAGGAGATAG
AGAATCTGAATGATTCTAAGCCCATTTCTGCCATCAACTTAACATCTTCCTGGACAATAA
CATCTATGAATGCAGAGCCAGAGACTTACGATAACTTCTTCAACAAAGAAGAGAGACAAA
CCTTGTATTTGTGATACCCATCACATGCTATATCTCCATTACTTCCATTATCTGCGATTT
TTGAACAATGATCTATACAATCTTCTCACAAAAACGCCGAATGATGACTCAAAGTTGAAA
ATAAAACAAAACAACTTACAACAGTGGGAGGTTGTATCCCAGACGCTAGGAGTTCTTCCG
TCTTCATTAGCAGCTCCTTCCCACTATTTCCACAAATTCCCCCAAAAAACTCAAATTGTA
GACAAAACAATTAAAAATGGAAAACCCCGATTGCCATTGATGTCCGGATATATTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
   
Sequence Name 21-K116452-0022-459-G12C-8409
GenBank Accession LN495813 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K116452-0022-459-G12C-8409
ATTGATATATCCGGACATCAATGGCAATCGGGGTTTTCCATTTTTATTGTTTTGTCTACA
TTTGAGTTTTTTGGGGGAATTTGTGGAAATAGTGGGAAGGAGCTGCTAATGAAGACGGAA
GAACTCCTAGCGTCTGGGATACAACCTCCCACTGTTGTAAGTTGTTTTGTTTTATTTTCA
ACTTTGAGTCATCATTCGGCGTTTTTGTGAGAAGATTGTATAGATCATTGTTCAAAAATC
GCAGATAATGGAAGTAATGGAGATATAGCATGTGATGGGTATCACAAATACAAGGTTTGT
CTCTCTTCTTTGTTGAAGAAGTTATCGTAAGTCTCTGGCTCTGCATTCATAGATGTTATT
GTCCAGGAAGATGTTAAGTTGATGGCAGAAATGGGCTTAGAATCATTCAGATTCTCTATC
TCCTGGTCAAGACTTATACCTAGTAACGCCTATTCTTGGTTTCTTAATTCCCAAGTAACT
AATAATGTTGTCTCTGACCTCTTATATTAGACCCTTTTTGTCTGTGTCATTTTTCAGATG
GAAGAGGACGCATTAACCCAAAAGGTTTATTGTTTTACAAGAATCTCATCAAAGAGCTAC
GAAGCCATGGTAAGGTTTTTGTCTTTTTACTTTTAACGGAACT

Last Updated on 06.12.2018 16:11