Sequences from confirmation amplicon


Confirmation sequences for line 460A03 and hit At1g55430 are shown.
Line ID 460A03
Locus At1g55430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20698992
Primers used for PCR GA03 - 8474, show primer details
   
Sequence Name 41-K026770-022-460-A03C-8409
GenBank Accession LN503941 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K026770-022-460-A03C-8409
TCCCGGACATGAAGCCATTTACAATTGAATATATCCTGGACATGAAATTTCACACTTGAA
CACACATATACACATAGACATGAAGCCACCTACCACTATCTCACACTTGAAATTTCATGA
GCACACCCTCACCCTCTTCCTTGTGCGAATCACTTGGCCATGCGATGTTTGTGGCTTTCT
TCTAAACAAAGACACCGACGATCTCGTTTACGCTTGTCTACCTTGCAGCCTTATGGTCCA
CAGATCATGTATCTATCTACCTCGTGTCATTAAGATCACCCGACACCCACATCGTCTCTC
TCTCACTTCCTCCCTTCAACCTGGTGATTTCTCTTGTGGACTTTGTCGCCATACTGTTGA
CGTTAACTGCGGGCAATACTCTTGTGATAAGGGATGCCAATATGCTATCCATTCCAAATG
TGCAACATGGAAAGACCTGTGGGAAGGAATAAATCTTGATGGAATGCCTG
   
Sequence Name 42-K026770-022-460-A03C-GA03
GenBank Accession LN504326 [GenBank]
Sequencing primer gene specific primer, GA03: 5'-ACAAAATCACACTCCATACATCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K026770-022-460-A03C-GA03
AATCTCTGAGAGATCATTACTGGAAGGGTGCATGCCTCACAAAACTTGTCTTTCTCGGTA
ATGGTTTTCTTTACATGGAGCTTCATATAATGCTCATGATGACTGAAATGTTGAATTGTG
TCCTCATCAATCTTCAGGAATGGCGGTTCGATATACTCCTCGGGTTCTTCAGGCACTCCA
TCAAGATCTATTCCATCCCACACGTCTCTCCATGTTGCACATTTCGAATGGATAGCATAT
TGGCATCCCTTATCACAAGAGTATTGCCCGCAGTTAACGTCAACAGTATGGCGACAAAGT
CCACAAGAGAAATCACCAGGTTGAAGGGAGGAAGTGAGAGAGAGACGATGTGGGTGTCGG
GTGATCTTAATGACACGAGGTAGATAGATACATGATCTGTGGACCATAAGGCTGCAAGGT
AGACAAGCGTAAACGAGATCGTCGGTGTCTTTGTTTAGAAGAAAGCCACAAACATCGCAT
GGCCAAGTGATTCGCACAAGGAAGAGGGTGAGGGTGTGCTCATGAAATTTCAAGTGTGAG
ATAGTGGTAGGTGGCTTCATGTCTATGTGTATATGTGTGTTCAAGTGTGAAATTTCATGT
CCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATT
ACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAA

Last Updated on 06.12.2018 16:11