Sequences from confirmation amplicon


Confirmation sequences for line 460B02 and hit At4g04610 are shown.
Line ID 460B02
Locus At4g04610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:2326486
Primers used for PCR HF69 - 8474, show primer details
   
Sequence Name 29-K101424-0022-460-B02C-8409
GenBank Accession LN499221 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K101424-0022-460-B02C-8409
CGGACTGAGCCATTTACATTGAAAGCCATGGAAGCATCGTATGATGAACTGGCGGATAAA
TTGGCTGGAAGTGGGATTAAGGTTGCCAAATTCAGAGCAGATGGTGACCAGAAGGAGTTT
GCTAAGCAGGAATTGCAGCTCGGTAGCTTCCCTACCATTCTGGTTTTCCCTAAGAACTCA
TCGAGACCGATCAAGTATCCGTCTGAGAAGAGAGATGTTGAGTCTTTGACTTCGTTCTTG
AATCTTGTCCGATAAGTAAACCACAAAACCAGTCGACATTTCTATGAGGAATAAGAATTA
TATCTTCGTCTCGTTCCAGATTGCTAGAACAGATGAAGTTGGTGTTTGGAGAATCAATTC
AAAGCTTTGGATCACAAAGTCAGAGATAAGCTCTGCTACAAAGCTTGAGTAATTGCAGTA
TTGTAAGATTTATAAAGTTGCGATAGTGTTGATTCTTCATGTAAATGTGTGGTGTCTATT
AAAATCAAGCCTCTGTTTCTTCTTTGCATTCATTTTTGAGAATATAGAGCTAAGAAACTG
ATGATTCTCCATGAAAATCAAGACCATGATTCTTCGTATTCATATCANATTATCATCATA
TACTAAGATACATTTCAAAGCTGAGATCAGGAACACTAAGATACATTTCANAGCTGAGAT
CTTCAGGAACACTAAGATACATTTTCAAGCTGAGATCTTCAGGAATCAGAACGAGTAAA
   
Sequence Name 69-K101427-0022-460-B02C-HF69
GenBank Accession LN512162 [GenBank]
Sequencing primer gene specific primer, HF69: 5'-CAGTTGCTTAGCATTGTTTACTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K101427-0022-460-B02C-HF69
CTTTGAATGTATCTAGTGTTCCTGAAGATCTCAGCTTTGAAATGTATCTTCGTTTTCCTG
ATCTCAGCTTTGAAATGTATCTTAGTATATGATGATAATTTGATATGAATACGAAGAATC
ATGGTCTTGATTTTCATGGAGAATCATCAGTTTCTTAGCTCTATATTCTCAAAAATGAAT
GCAAAGAAGAAACAGAGGCTTGATTTTAATAGACACCACACATTTACATGAAGAATCAAC
ACTATCGCAACTTTATAAATCTTACAATACTGCAATTACTCAAGCTTTGTAGCAGAGCTT
ATCTCTGACTTTGTGATCCAAAGCTTTGAATTGATTCTCCAAACACCAACTTCATCTGTT
CTAGCAATCTGGAACGAGACGAAGATATNATTCTTATTCCTCATAGAAATGTCGACTGGT
TTTGTGGTTTACTTATCGGACAAGATTCAAGAACGAAGTCAAAGACTCAACATCTCTCTT
CTCAGACGGATACTTGATCGGTCTCGATGAGTTCTTAGGGAAAACCAGAATGGTAGGGAA
GCTACCGAGCTGCAATTCCTGCTTAGCAAACTCCTTCTGGTCACCATCTGCTCTGAATTT
GGCAACCTTAATCCCACTTC

Last Updated on 06.12.2018 16:11