Sequences from confirmation amplicon


Confirmation sequences for line 460E02 and hit At5g14610 are shown.
Line ID 460E02
Locus At5g14610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4713487
Primers used for PCR 41ve - 8474, show primer details
   
Sequence Name 62-K117894-0022-460-E02C-41ve
GenBank Accession LN510846 [GenBank]
Sequencing primer gene specific primer, 41ve: 5'-GCGATGCAAAACAGAGACATAGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K117894-0022-460-E02C-41ve
GAACTTTGGGTACTTGATTCCTGGTTTCTGCATCTCCACGGATCCACAATGATTCACGAA
TGGGCCCAACGATCTTGGTCTTGTCCCCTACTAGGGAGTTGGCCACACAAATCCAAGTTG
AAGCTCTGAAATTTGGCAAGTCATCTAAAATTTCGTGTGCGGTAAGTGAAAATACTTGAA
TACATAAGTACTGAACATGCTACTTGAGAAGTGTCTTATTGAGTGTTTTTGTCAATGTAT
ATTTGTAGTGTTTGTATGGTGGAGCACCCAAGGGTCCTCAGCTGAAGGAAATAGAAAGAG
GTGTAGATATTGTTGTTGCAACTCCCGGGCGACTGAATGATATCCTTGAAATGAAGAGAA
TCAGTCTGCATCAAGTATCTTACCTTGTGCTGGACGAGGCAGATAGAATGCTGGACATGG
GGTTCGAACCCTCATGAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAAATGTCCACAAAGTTATA
   
Sequence Name 51-K117893-0022-460-E02C-8409
GenBank Accession LN507627 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K117893-0022-460-E02C-8409
AATTGAATAATCCTCATGAGGGTTCGAACCCCATGTCCAGCATTCTATCTGGCTCGTCAA
GCACAAGGTAATATACTTGATGCAGACAGATTCTCATCATTTCAAGGATATCATTCAGTC
ACCCGGGAGTTGCAACAACAATATCTACACCTCTATCTATTTCCTTCAGCTGAGGACCCT
TGGGTGCTCCACCATACAAACACTACAAATATACGTTGACTATAACACTCAATAATACAC
TTCTCAAGTAGGGGGGC

Last Updated on 06.12.2018 16:11