Sequences from confirmation amplicon


Confirmation sequences for line 461C07 and hit At1g77280 are shown.
Line ID 461C07
Locus At1g77280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29034092
Primers used for PCR 49ye - 8474, show primer details
   
Sequence Name 07-K121121-0022-461-C07C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K121121-0022-461-C07C-8409
CTATAAGCGGAAAATTCACATTTCGTTTCTCTTCTGAAGAAGCTAAAGAAGTAAGCAAAC
TAGCAAAGGAACAAAATTGAAAAAATCCAGACTTTGATCTTATTTTACCTGTTTCAAATT
GCAGAAACCTTCATAAACGTCAAGAACAGAGTCGAAAGTTCTAACCAGAGAGAGAAGCGA
AGAGTTCCCTGCGCGATCCACAATCTCTAAACAAAACCCAAAACCGAAACTTTTGTGTTA
ACAAAAACATAAAAATCAAAGCTTTTTGGTAAGAGAAATTATATTAAGGAAAAGCTCAGA
AATGTAACCATTTCCAAGGATATGAAGAGCGATCACAGTATCACCAGGCTCAGCGACTTT
AACCAGAGCCCAAGTGAGTAACTCTCTGCTCGGAGCGTCAAGCTTCACGCCGACAAGAAT
CGTACGGCCACCACATCCTTCCTCGACGGAGCTCTGTTTATCTCCGCCGGTTTCTATCAT
CTCTGATTGTTCTGAATTTTGATTTCAAGTGTGTCCTTTTATCCTCCTGACGTGTGCTTC
ACAAAGACAAAAGAACATCGTTTTTAAGGATTTATATAACCAAACAAAGACAAGATCTTT
CTCTCTCTCTCTATCTACCTGCAGAGACTTAAGTTTTAACCGCAGGTTTGGTTTTGCTTT
TGCTTTTGATGCTCTCGTCGTCACAAAGAAAGAGAAGAGCAGCCATCTTATTAGATTTTC
ATTTTCCTTTTTCTTAAAACCCCACTTGTACTTCTTTTTTTTAACTCGCTTTTTTGTAAA
TTCATTTGTTTTTGCATTTTGACATAGTCTTACTATGGCATTGAATCACAATGACAGATA
CAGTTATGTTCTTATTATTTGCTAGGGTAATAAATATCAAAACTAAATGTCTCTGCT
   
Sequence Name 23-K121122-0022-461-C07C-49ye
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 49ye: 5'-CTTTATTTACACCGACCCATTCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K121122-0022-461-C07C-49ye
TTGACTTCATTTTAGTCAACAAAATAGTTGCAAACATTGTATCTTTCTATTGTCGTATTA
TTTCTTGTTTTGGCTTTTTTTTAGCCAAACAATATATTCTCCATACTATTTTTGAGGAAT
CTGTTTTTAGCTTAAATAGATGATTTTTTGCTGTAAAAACAATATATATATTTTTAATCT
AAACTATTTTAGCATAATGATTAAAGTAGTTTAAGCTCTAAAGTAGGGAACTGAACCATA
CAATCAACGTTCCAACTATTTCCACATACATAGTAGTTTTGTACGGCTTTTCAGGAGAAA
GCAGAGACATTTAAGTTTTGATAATTTATTAACCTTAGCAAATTAATAAGAACATAACTG
TATCTGTCATTGTGATTCAATGCCATAGTAAGACTATGTCAAAATTGCAAAACAAATGAA
TTTACAAAAAAGCGAGTTAAAAAAAAGAAGTACAAGTGGGGTTTTAAGAAAAAGGAAAAT
GAAAATCTAATAAGATGGCTGCTCTTCTCTTTCTTTGTGACGACGAGAGCATCAAAAGCA
AAAGCAAAACCAAACCTGCGGTTAAAACTTAAGTCTCTGCAGGTAGATNGAGAGAGAGAG
AAAGATCTTGTCTTTGTTTGGTTATATAAATCCTTTAAAACGATGTTCTTTTGT

Last Updated on 06.12.2018 16:11