Sequences from confirmation amplicon


Confirmation sequences for line 461D10 and hit At4g28710 are shown.
Line ID 461D10
Locus At4g28710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14173124
Primers used for PCR CC43 - 8474, show primer details
   
Sequence Name 54-K106193-0022-461-D10C-CC43
GenBank Accession LN508486 [GenBank]
Sequencing primer gene specific primer, CC43: 5'-TGGAACATGAATTTACCATTTGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K106193-0022-461-D10C-CC43
TTGATCTGGGGATCAGGTTCTAGTTCATCCATTTAGGGGTTCTAGTTCTTTGACACAAGT
GTAGTACCTAGAGTATAGAATGGTCCTCTTTCGGAGAGGGATTCTTCTTATTGTAACTTT
AGTTTTGAACTGTTTTTAGTTTCTTGTTGTAGCAGATATGGCTTGTTACTAGTTTGAAAG
GGAATATACTCATACTATGTTTTAGAAGTTGAAATAAAGCTGTGAAAATAGAAGTATCTG
AATTGTTGGTTTTGCAGGCTTGTACTACAGTCAATGTTGGCTCTTGCGTTTGGGTGGAAG
ATCCAGAAGTGGCATGGATAGACGGTGAAGTTATCGAGGTTAAAGGAAGTGATATTAAGG
TCAAATGTACTTCAGGGAAGACGGTATGTTTTACCAGTTTGAATCATTATTTACCCATCA
ACTTCTCAATTTTGAAATTTTACGTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCC
   
Sequence Name 70-K106194-0022-461-D10C-8409
GenBank Accession LN512395 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K106194-0022-461-D10C-8409
CATTTACCGTAAAATTTCAAAATTGAGAAGTTGATGGGTAAATAATGATTCAAACTGGTA
AAACATACCGTCTTCCCTGAAGTACATTTGACCTTAATATCACTTCCTTTAACCTCGATA
ACTTCACCGTCTATCCATGCCACTTCTGGATCTTCCACCCAAACGCAAGAGCCAACATTG
ACTGTAGTACAAGCCTGCAAAACCAACAATTCAGATACTTCTATTTTCACAGCTTTATTT
CAACTTCTAAAACATAGTATGAGTATATTCCCTTTCAAACTAGTAACAAGCCATATCTGC
TACAACAAGAAACTAAAAACAGTTCAAAACTAAAGTTACAATAAGAAGAATCCCTCTCCG
AAAGAGGACCATTCTATACTCTAGGTACTACACTTGTGTCAAAGAACTAGAACCCCTAAA
TGGATGAACTTAGAACCTTGATCCCCAGATCAAAAGATTTAGAGGATCATATTCAAATGG
TAAATCANTGTTCC

Last Updated on 06.12.2018 16:11