Sequences from confirmation amplicon


Confirmation sequences for line 461D10 and hit At5g64990 are shown.
Line ID 461D10
Locus At5g64990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25963756
Primers used for PCR 16aj - 8474, show primer details
   
Sequence Name 14-K106680-0022-461-D10C-8409
GenBank Accession LN492523 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K106680-0022-461-D10C-8409
TTGAATAATATTTGTGAAGCTGCCTCGTTTCCTTGTCTCTATTGACTTGTGTGTGTGTGT
TTTTGTTTGTTTACCTTAATGTTGAATCCAGCTTTAGCACTAGTCTCCATGAACAAAGCT
CCAAACTCACGAGCTTTATTCTCCCCTTCCTCTATCGAAACTTGCCTATAGTTTAATCAA
TCACATCACACAACATCATATGGGGTTTGTGAAATGAAGAAGCTATCTTTTGATTTGATT
TTAATGGACATAGTTATACCTTTTATTAACAAGATCGGTTTTGTTACCAACAAGAACAAT
AATGACATAGCTGCCTCTTTCTGCACGAACTTCTTCGATCCACTTGGAGGTGTTTATAAA
TGATTGCTTACCTGCACATATATAACTTTTGTAAGAAGAAAACGAGCATGATGAGTGGTT
ATGAAACTATTTTTTTGTTATAGAGTAAATAAGTCGCATAACTGGCAACATCATAGACAA
TAACAGCAACAGAAGAATCTCTGATATAACTTGGTACTAGGCTTTTAAATCTCTCTTGTC
CTGCAGTATCCCTACAAGATAATCAACACAAACACGAAGACATTTTCATAG
   
Sequence Name 30-K106681-0022-461-D10C-16aj
GenBank Accession LN499735 [GenBank]
Sequencing primer gene specific primer, 16aj: 5'-GCTATGAAAATGTCTTCGTGTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K106681-0022-461-D10C-16aj
CCTAGTACCAAGTTATATCAGAGATTCTCCTGTTGCTGTTATTGTCTATGATGTTGCCAG
TTATGCGACTTATTTACTCTATAACAAAAAAATAGTTTCATAACCACTCATCATGCTCGT
TTTCTTCTTACAAAAGTTATATATGTGCAGGTAAGCAATCATTTATAAACACCTCCAAGT
GGATCGAAGAAGTTCGTGCAGAAAGAGGCAGCTATGTCATTATTGTTCTTGTTGGTAACA
AAACCGATCTTGTTAATAAAAGGTATAACTATGTCCATTAAAATCAAATCAAAAGATAGC
TTCTTCATTTCACAAACCCCATATGATGTTGTGTGATGTGATTGATTAAACTATAGGCAA
GTTTCGATAGAGGAAGGGGAGAATAAAGCTCGTGAGTTTGGAGCTTTGTTCATGGAGACT
AGTGCTAAAGCTGGATTCAACATTAAGGTAAACAAACAAAAACACACACACACAAGTCAA
TGTGATACAAGGAAACGAGGCAGCTTCACAAATATATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGGTTATT

Last Updated on 06.12.2018 16:11