Sequences from confirmation amplicon


Confirmation sequences for line 462A04 and hit At5g55550 are shown.
Line ID 462A04
Locus At5g55550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22502798
Primers used for PCR NU29 - 8474, show primer details
   
Sequence Name 12-K103931w-0022-462-A04C-NU29
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, NU29: 5'-CCATTGTAAGTTTCTGGGTCATTT-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K103931w-0022-462-A04C-NU29
CATATCTAACCGTAAGCGCGAGACAAATTGTCTAGCTCGTGAGAACACTGAGATGGTAGC
TGCTGTTGCTGCCACGTTGAGTCGCTGTAGACTGAGCCGCCTCTGTACAGTTCCCCTATA
GAACCTTCAAAACCATTAAATGGGGACGAGGAAAGTCCAGAACTTGTCCCATAGTTCCTT
CCAAAGCCATTGTTATCATTACTAACAGCAGAAGAAGAAGGAAGTCCCCAATTTCCTCTG
TTGTTTCCATTCGAGACATTCAAGTTCCAACCGGGACCTGCAGTGTCGGTTCTGTTTCCC
CATAAGTCTCTATTGTTCGAATTGTAAGGAGACTCAGTTCTATTGTGCCCGATTGGAGAG
GTGTAACGGTTCGGGGAAGCACCGTTGAAGTAAGGGTTGCTTGGAATACGGTTATAACCA
AACGTAGAACTTGTCCCATCGCAGCTTGGATTCAAATTCAAACTCAAGTCATGATTCAAA
CCGAGGCCAAAAGCAGAAACCGCATTTCTACCACTACCAATAACAGGATATATTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACG
GTTAT
   
Sequence Name 28-K103932w-0022-462-A04C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K103932w-0022-462-A04C-8409
TTGAATATATCCTGTTATTGGTAGTGGTAGAAATGCGGTTTCTGCTTTTGGCCTCGGTTT
GAATCATGACTTGAGTTTGAATTTGAATCCAAGCTGCGATGGGACAAGTTCTACGTTTGG
TTATAACCGTATTCCAAGCAACCCTTACTTCAACGGTGCTTCCCCGAACCGTTACACCTC
TCCAATCGGGCACAATAGAACTGAGTCTCCTTACAATTCGAACAATAGAGACTTATGGGG
AAACAGAACCGACACTGCAGGTCCCGGTTGGAACTTGAATGTCTCGAATGGAAACAACAG
AGGAAATTGGGGACTTCCTTCTTCTTCTGCTGTTAGTAATGATAACAATGGCTTTGGAAG
GAACTATGGGACAAGTTCTGGACTTTCCTCGTCCCCATTTAATGGTTTTGAAGGTTCTAT
AGGGGAACTGTACAGAGGCGGCTCAGTCTACAGCGACTCAACGTGGCAGCAACAGCAGCT
ACCATCTCAGTCTTCTCACGAGCTAGACAATTTGTCTCGCGCTTACGGTTATGATATTGA
CAATGTAGGTTCAGACCCATCTGCAAATGACCCAGAAACTTACAATGG

Last Updated on 06.12.2018 16:11