Sequences from confirmation amplicon


Confirmation sequences for line 464A10 and hit At3g12910 are shown.
Line ID 464A10
Locus At3g12910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4110339
Primers used for PCR 39hw - 8474, show primer details
   
Sequence Name 53-K112667-0022-464-A10C-39hw
GenBank Accession LN508201 [GenBank]
Sequencing primer gene specific primer, 39hw: 5'-ACTAAGAAAAGAAGGTGGGAAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K112667-0022-464-A10C-39hw
GGCCAGTCTCGTAGGTTTCATGGTGAATGTTATCGTTGGAGAAATGTTGAAACGAATCAA
CGTGTGTGGTGTCCATGAGAATTGGCTGGTTTTGATGTTGATAAGGCGTTTGCTGAAGCT
GGAAAACGCTTCTCTCGTAGTTCCCTTTGCCTTCCCTAACGTTCATCACGTTGGTACTAC
TACTACTTTCATTGTCCCCAAAATTTATATAAGCTTCTTGGTAATTAGATTGTTGCTGCT
TCACACGTTTGCCATTTGCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 21-K112666-0022-464-A10C-8409
GenBank Accession LN495732 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K112666-0022-464-A10C-8409
TGGCAAACGTGTGAAGCAGCAACAATCTAATTACCAAGAAGCTTATATAAATTTTGGGGA
CAATGAAAGTAGTAGTAGTACCAACGTGATGAACGTTAGGGAAGGCAAAGGGAACTACGA
GAGAAGCGTTTTCCAGCTTCAGCAAACGCCTTATCAACATCAAAACCAGCCAATTCTCAT
GGACACCACACACGTTGATTCGTTTCAACATTTCTCCAACGATAACATTCACCATGAAAC
CTACGAGACTTGGCCGGACGAGCTTCGATCGGTTGTGGAATTTGCTTTCCCACCTTCTTT
TCTTAG

Last Updated on 06.12.2018 16:11