Sequences from confirmation amplicon


Confirmation sequences for line 464A10 and hit At5g41800 are shown.
Line ID 464A10
Locus At5g41800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16734151
Primers used for PCR 43vd - 8474, show primer details
   
Sequence Name 32-K117776-0022-464-A10C-43vd
GenBank Accession LN500746 [GenBank]
Sequencing primer gene specific primer, 43vd: 5'-ATTCCAGTGTTGATAGCTGTTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K117776-0022-464-A10C-43vd
CATCCGATCCTGCACACATCACAATAACCGGGCTAAAATTCGAAATCGGCCCTTAAACCC
AAAACTCAATAACCGGAAAGACCAGACTTTATGACGTAAACCGATCTATAAACCGAAATA
CACAGGCCTAACCTAGACCGGTACGGTAAACTAAGACACGTGTGATGATGGCGAATCTAA
CAAGAAGTCAAAGTATCCAAATCGAAACACGATCCAACCTTTACGGATTTATCCAAAAAA
AGCAAATTAATAAATACAAAGAAGAGATTTTGAATGATACCAAGAACATCGGCGGCGAGT
TCCCGGAAACGGATGTGACGGCGGCCGGATTTTTCACAGTGATCAAGAACCTTAGACATG
AGGTAATAATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGATT
ATTAT
   
Sequence Name 08-K117775-0022-464-A10C-8409
GenBank Accession LN489916 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K117775-0022-464-A10C-8409
CTCTGTCTAGGTTCTTGATCCTGTGAAAAATCCGGCCGCCGTCACATCCGTTTCCGGGAA
CTCGCCGCCGATGTTCTTGGTATCATTCAAAATCTCTTCTTTGTATTTATTAATTTGCTT
TTTTTGGATAAATCCGTAAAGGTTGGATCGTGTTTCGATTTGGATACTTTGACTTCTTGT
TAGATTCGCCATCATCACACGTGTCTTAGTTTACCGTACCGGTCTAGGTTAGGCCTGTGT
ATTTCGGTTTATAGATCGGTTTACGTCATAAAGTCTGGTCTTTCCGGTTATTGAGTTTTG
GGTTTAAGGGCCGATTTCGAATTTTGAGCCGGTTATTGTGATGTGGTGCAGGATCTGGAT
TGATGTTTTATGTAGTGATCTTTATTCAAACAGCTATCAACACTGGAAT

Last Updated on 06.12.2018 16:11