Sequences from confirmation amplicon


Confirmation sequences for line 466C11 and hit At2g41180 are shown.
Line ID 466C11
Locus At2g41180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17165622
Primers used for PCR 68lx - 8474, show primer details
   
Sequence Name 35-K115914-0022-466-C11C-68lx
GenBank Accession LN501960 [GenBank]
Sequencing primer gene specific primer, 68lx: 5'-CTAACTCATGTGTTTAGAACAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K115914-0022-466-C11C-68lx
ATCGTTCAGGTTTTTTTTCTTCTTCTCTTTACATCGAACCATAGCTCACAAATCACAATT
CTAGAGGAATTTAAAAATTGTGAAGCGTTGAATATTTCTACAATTTAAGATATCACAATA
GTCAACTATACGATAAACATTTCGTACGAAATAAGTTGTTGTTTTCATTAAATGTTGTTT
GTATCAAACGAATATTTCCATAAGATAGTGTGTTTGTAGATTGTAAATATAAAATAATGT
GATTAATAAATGATAAGAAAATAACAAAACTCTTCTATCATTCATTCCAAATTTCCAAAA
TATAAATGTATTATTGCGTCTATTATACAGAGATGGGGTCATCCTAAAAAACAAAAGGGA
TACATCATACATCTATCCAAATGTTCAACTAAAGTCCGTGATAACTTAAAAGCTCTATAT
GGATTATTATTTCTTTTCCGTTTGTGATCATTTACACCAGAGATTAAACTGATCACAAAA
TCACTACCACCAAATTGGGATTTTGAGGTTAATTATTACATAACTACACCAATAAACCCA
ACTAAAACTAATACGGTTTGATTATTATTATAAAATAAAAAAACCACAAACAAATGAAAG
ATTTCTATATATACTCACAAACGTAAATAATTCGCGAGAAAAAAAACTATATATGTAATA
TATCCATATATAGGTTTTGATGAAAACCCTAAAACTATAGATGGTTTATTCTTCGTGATC
GGTGTTTCAGTGCCGAAACAAAACATTCATAGTTTAAAATTCAGAGAGAACCAATGCTTC
CTAAAGCGTTAACATTGTAAAATCCACAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 09-K115913-0022-466-C11C-8409
GenBank Accession LN490336 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K115913-0022-466-C11C-8409
ATTTCATTGTGGATTTTACAATGTTACGCTTTAGGAAGCATTGGTTCTCTCTGAATTTTA
ACTATGAATGTTTTGTTTCGGCACTGAAACACCGATCACGAAGAATAAACCATCTATAGT
TTTAGGGTTTTCATCAAAACCTATATATGGATATATTACATATATAGTTTTTTTTCTCGC
GAATTATTTACGTTTGTGAGTATATATAGAAATCTTTCATTTGTTTGTGGTTTTTTTATT
TTATAATAATAATCAAACCGTATTAGTTTTAGTTGGGTTTATTGGTGTAGTTATGTAATA
ATTAACCTCAAAATCCCAATTTGGTGGTAGTGATTTTGTGATCAGTTTAATCTCTGGTGT
AAATGATCACAAACGGAAAAGAAATAATAATCCATATAGAGCTTTTAAGTTATCACGGAC
TTTAGTTGAACATTTGGATAGATGTATGATGTATCCCTTTTGTTTTTTAGGATGACCCCA
TCTCTGTATAATAGACGCAATAATACATTTATATTTTGGAAATTTGGAATGAATGATAGA
AGAGTTTTGTTATTTTCTTATCATTTATTAATCACATTATTTTATATTTACAATCTACAA
ACACACTATCTTATGGAAATATTCGTTTGATACAAACAACATTTAATGAAAACAACAACT
TATTTCGTACGAAATGTTTATCGTATAGTTGACTATTGTGATATCTTAAATTGTAGAAAT
ATTCAACGCTTCACAATTTTTAAATTCCTCTAGAATTGTGATTTGTGAGCTATGGTTCGA
TGTAAAGAGAAGAAGAAAAAAAACCTGAACGATTTATGAGATAGCCATTTTCAATTCAAA
AATATTTTGTTCT

Last Updated on 06.12.2018 16:11