Sequences from confirmation amplicon


Confirmation sequences for line 467E12 and hit At2g42740 are shown.
Line ID 467E12
Locus At2g42740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17791926
Primers used for PCR HF62 - 8474, show primer details
   
Sequence Name 88-K101272-0022-467-E12C-8409
GenBank Accession LN514839 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K101272-0022-467-E12C-8409
CGGAATGAGCCTTTACATTGAATATATCCTGGTTCTCAACATCTCCGTTGGTGAGAGTGG
AGATCGTCTCACCAGAGCCTCCAAGGTTCCTCCTGTTTTTTACCCATTCTCTGTGGTTTT
CACTCTTGTTGTCACTTGGTTATAATCGATTGATTCGTGGTTACTTGGTAGGTTTTGGAA
CAATTGAGTGGTCAGACTCCTGTTTTCTCCAAAGGTTAGATTTTTTCTCATATTTGAAAG
CTTACAAATAGTCTGGTTGATAGATTTAGTAATCTAGAAGAATTTGGGAAACATAGTTTA
GTTAAGTCTATGAATGGGTGCATGGTTTGATGATGAATTTGAGATTTTGGAATCCAGTAA
GTCCCTTATCTGGTGGTTTGTGATAACTTGATAAGCCTACCAGTCCAAATTAGGAGCTGT
TCGTTTCCCCGCTTCTGATATAGTTGCGTTGTGCACTCTGTTTTATATGCATCG
   
Sequence Name 88-K101278-0022-467-E12C-HF62
GenBank Accession LN514840 [GenBank]
Sequencing primer gene specific primer, HF62: 5'-CAAAAGCGATGCATATAAAACAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K101278-0022-467-E12C-HF62
GGGGAACGAAAGCTCCTAATTTGGACTGGTAGGCTTATCAAGTTATCACAAACCACCAGA
TAAGGGACTTACTGGATTCCAAAATCTCAAATTCATCATCAAACCATGCACCCATTCATA
GACTTAACTAAACTATGTTTCCCAAATTCTTCTAGATTACTAAATCTATCAACCAGACTA
TTTGTAAGCTTTCAAATATGAGAAAAAATCTAACCTTTGGAGAAAACAGGAGTCTGACCA
CTCAATTGTTCCAAAACCTACCAAGTAACCACGAATCAATCGATTATAACCAAGTGACAA
CAAGAGTGAAAACCACAGAGAATGGGTAAAAAACAGGAGGAACCTTGGAGGCTCTGGTGA
GACGATCTCCACTCTCACCAACGGAGATGTTGAGAACCAGGATATATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGAT

Last Updated on 06.12.2018 16:11