Sequences from confirmation amplicon


Confirmation sequences for line 467G04 and hit At5g25250 are shown.
Line ID 467G04
Locus At5g25250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8749589
Primers used for PCR IW60 - 8474, show primer details
   
Sequence Name 32-K033212-022-467-G04C-IW60
GenBank Accession LN500478 [GenBank]
Sequencing primer gene specific primer, IW60: 5'-CTTAAACTCCTTTGTCCCTTCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K033212-022-467-G04C-IW60
CTTTTACAAACTTTCCAAAATATGTCTTAGTTATAACCAATCAAACTGACATTTATTTAA
AGAGACTTAGCCAAATCATTATACAAAACCCTAGTCTTACTACAACTTTATGTCACCAAT
CAAATCCGAAAAATATCTACATAAATTAAATCTACCTTAACATCCTCATATTCACCAATA
AACTTAACATCTAAAACCCTAAATTTGGGGTTTACCTTTAAAGATCTCTTCCATAGTCAT
GGAAGCAGCAAGAACACGAGTCTCTCCTTCAATAACACCTTCAACAAGCTCATGGACATG
ATTTGAATCTTTATCATGAGGAGAAATCAACCTAGCATACAAAATCAGCGCGTCATCGTC
ATCAACGCGAGGACCAATCGTAAAAACAGCGGGAAGAACAAAAGGAAGCTTCTCTGCGCT
CATGGCTTGGACTTTGAAAGTATAGTTCACCGGAGAAACGTCGAAGACGGTGCAAGATTG
CCATGGAAACACCCATGACTTCTTGGAAAGCTTGATGTCTTCGATACCAGCACCGGTGAT
CGCCAAATACTGTGACGCTCTTGCAACTTTGAACATCTTTTGATTTAATTTGGACTTTTC
GCCCAAATGATTTAGAAGACTTTGTTTTGAGTTCTTAAGGACTTATTATATATATATAGT
CGTGCGTTATGTACGAAGACTTAGTCTTACAGAAAAAATCAACGCATTTCCAAGAAACTA
CATATTTGAAGCCATCGCCTATTGGCGGCGAGGTACTTTAGATTACCAATAGGATATATT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGT
ATTTATAGGCGAAAAGCATAAACAAATT
   
Sequence Name 24-K033212-022-467-G04C-8409
GenBank Accession LN496898 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K033212-022-467-G04C-8409
GCCTTTACAATTGAATATATCCTATTGGTAATCTAAAGTACCTCGCCGCCAATAGGCGAT
GGCTTCAAATATGTAGTTTCTTGGAAATGCGTTGATTTTTTCTGTAAGACTAAGTCTTCG
TACATAACGCACGACTATATATATATAATAAGTCCTTAAGAACTCAAAACAAAGTCTTCT
AAATCATTTGGGCGAAAAGTCCAAATTAAATCAAAAGATGTTCAAAGTTGCAAGAGCGTC
ACAGTATTTGGCGATCACCGGTGCTGGTATCGAAGACATCAAGCTTTCCAAGAAGTCATG
GGTGTTTCCATGGCAATCTTGCACCGTCTTCGACGTTTCTCCGGTGAACTATACTTTCAA
AGTCCAAGCCATGAGCGCAGAGAAGCTTCCTTTTGTTCTTCCCGCTGTTTTTACGATTGG
TCCTCGCGTTGATGACGATGACGCGCTGATTTTGTATGCTAGGTTGATTTCTCCTCATGA
TAAAGATTCAAATCATGTCCATGAGCTTGTTGAAGGTGTTATTGAAGGAGAGACTCGTGT
TCTTGCTGCTTCCATGACTATGGAAGAGATCTTTAAAGGTAAACCCCAAATTTAGGGTTT
TAGATGTTAAGTTTATTGGTGAATATGAGGATGTTAAGGTAGATTTAATTTATGTAGATA
TTTTTCGGATTTGATTGGTGACATAAAGTTGTAGTAAGACTAGGGTTTTGTATAATGATT
TGGCTAAGTCTCTTTAAATAAATGTCAGTTTGATTGGTTATAACTAAGACATATTTTGTG
AAAGTTTTGGTTAAAAGAGTTTTTGATTTTATGTATTTGAAGGGACAA

Last Updated on 06.12.2018 16:11