Sequences from confirmation amplicon


Confirmation sequences for line 467G10 and hit At3g46340 are shown.
Line ID 467G10
Locus At3g46340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17030854
Primers used for PCR EQ76 - 8474, show primer details
   
Sequence Name 35-K103355-0022-467-G10C-EQ76
GenBank Accession LN501796 [GenBank]
Sequencing primer gene specific primer, EQ76: 5'-GTGTACAGGTCGAACTTTTGTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K103355-0022-467-G10C-EQ76
GTATTGTGATGAAGAGATCACTTGGCTCTCATCTATGAATACATGTCGAATAAAGACTTA
AAACATCATTTGTCAGGTGAAAGATTGTATAACAGTTACATACATATATTTCTTCTAACA
GAAAAAAGCGAATATGTGATTTTACAATCTACTGTAGGAAAACACGGTGGCTCTGTTTTA
AAGTGGAATACCCGACTACAAATAGCCGTCGATGCCGCACTAGGTTTGAACTATCCCAAA
ATAATTACATTCATGTGTTTTTACTATCACAAAATATTTTATGTGGGATTAGTGGGCACA
AATGATTGAAATATTAAATGGTACTATATTCTTAGTATTACTATTTTTGTTTGTTTAATT
TTGGTCAACTAATTGTTCTTATTGTTAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 51-K103356-0022-467-G10C-8409
GenBank Accession LN507471 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K103356-0022-467-G10C-8409
CATTTACAATTAACAATAAGAACAATTAGTTGACCAAAATTAAACAAACAAAAATAGTAA
TACTAAGAATATAGTACCATTTAATATTTCAATCATTTGTGCCCACTAATCCCACATAAA
ATATTTTGTGATAGTAAAAACACATGAATGTAATTATTTTGGGATAGTTCAAACCTAGTG
CGGCATCGACGGCTATTTGTAGTCGGGTATTCCACTTTAAAACAGAGCCACCGTGTTTTC
CTACAGTAGATTGTAAAATCACATATTCGCTTTTTTCTGTTAGAAGAAATATATGTATGT
AACTGTTATACAATCTTTCACCTGACAAATGATGTTTTAAGTCTTTATTCGACATGTATT
CATAGATGAGAGCCAAGTGATCTCGTTCATCACAATACCCTACAAGGCTTACCAAGTTTA
TGTGGTGAACTCTCAACAAAAGTTCACCCTGTACA
Primers used for PCR 70xb - 3144, show primer details
   
Sequence Name 70-K118874-0022-467-G10C-70xb
GenBank Accession LN512474 [GenBank]
Sequencing primer gene specific primer, 70xb: 5'-TCAATCACACGTTGGTTTGTTATT-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K118874-0022-467-G10C-70xb
CGCTCATTTCAGCTACCGACCTGTTCTGTAATATCTGGAGAGGCAAGAATATGAGGTACA
ATTAATATGTGTTGGTTGTACCACTAATAGTAAGGACAACGTTCTAAAACTTTTGTGTCC
ATTTTCATTATAGGTCATTCCATATCTCTGGTATCCGGTTTGAGAGGCTGTAAGGACCTA
AGCTCAGAACCTCTTAATAATTCGAAAAAAGAAAAATACTTACTCGGGGTCAAGATATCC
AGGAGTACCAGCAACAACGGTTGAAACCTGAGATTCATCTCCGAGTTGGAAAGATCTTGA
CAGCCCGAAATCCGCCATTTTAGCAGTAAATTGATCGTCCAACAATATATTTGTACTTTT
GACATCTCTATGCACCATCGATGGTCGACATCCAATATGTAAGTATTCCAGTCCTTTAAT
TTATCAGATCAGGAAAAAGCCTTTCGGTATTAATTCTGATTGTGTGATTATTTTAACTAA
TCTTAGTAAGAATCAGAAGAAAAACGTTTTAAACGAAAAATATCTAAACCTTCTAAATTT
TAAAAACTATACTTTCATCCTTATAGAAAGTTTATGAAAATGTGGAATACTTTATTGCAA
CGATAAGAGCATATAGACGTTCATTAAACACTGATAGTTTAAACCGAAGGCGGGAAACGA
CAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGA
AGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTAC
   
Sequence Name 30-K118873-0022-467-G10C-3144
GenBank Accession LN499931 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K118873-0022-467-G10C-3144
CCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACC
CGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTAATGAACGTCT
ATATGCTCTTATCGTTGCAATAAAGTATTCCACATTTTCATAAACTTTCTATAAGGATGA
AAGTATAGTTTTTAAAATTTAGAAGGTTTAGATATTTTTCGTTTAAAACGTTTTTCTTCT
GATTCTTACTAAGATTAGTTAAAATAATCACACAATCAGAATTAATACCGAAAGGCTTTT
TCCTGATCTGATAAATTAAAGGACTGGAATACTTACATATTGGATGTCGACCATCGATGG
TGCATAGAGATGTCAAAAGTACAAATATATTGTTGGACGATCAATTTACTGCTAAAATGG
CGGATTTCGGGCTGTCAAGATCTTTCCAACTCGGAGATGAATCTCAGGTTTCAACCGTTG
TTGCTGGTACTCCTGGATATCTTGACCCCGAGTAAGTATTTTTCTTTTTTCGAATTATTA
AGAGGTTCTGAGCTTAGGTCCTTACAGCCTCTCAAACCGGATACCAGAGATATGGAATGA
CCTATAATGAAAATGGACACAAAAGTTTTAGAACGTTGTCCTTACTATTAGTGGTACAAC
CAACACATATTAATTGTACCTCATATTCTTGCCTCTCCAGATATTACAGAACAGGTCGGT
TAGCTGAAATGAGCGATGTCTACAGTTTTGGAATTGTGTTATTGGAGATAATAACAAAC

Last Updated on 06.12.2018 16:11