Sequences from confirmation amplicon


Confirmation sequences for line 468D06 and hit At5g43640 are shown.
Line ID 468D06
Locus At5g43640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17531567
Primers used for PCR 20xp - 8474, show primer details
   
Sequence Name 33-K119546-0022-468-D06C-20xp
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 20xp: 5'-ATATTTTTAGGGTTACAGGATGCG-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K119546-0022-468-D06C-20xp
TGTCTTTGCGCATACATTGTTTTATTTTGAGGCTTTGTGGATTTTTTAGGGTTTCATCGA
TCCAACAATGGTCAGTTCCAATCTTTGTGGATATGGAACCAGAGGTTGTTGCGGCTGGAA
TTGTGAAAAAGAGAACCTTCAAAAAGTTTTCTTTCAGAGGAGTCGATCTCGATGCGCTTC
TCGACATGTCCATCGAAGATCTTGTCAAACATTTCAGTTCCCGTATTCGCAGAAGGTTAC
CTTCTTTCTTTGAACCAAACAAAAGCTTTTGTCTAATTGGTTTTGTGAGACTGATGATGT
GTTTTGTTGTCTTTAAAGGTTTTCTAGAGGATTGACCAGAAAACCTATGGCTTTGATTGG
CTCAGCCATATATGGATTTCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 01-K119545-0022-468-D06C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K119545-0022-468-D06C-8409
TCGGATGAGCATTTCATTGAATATATCCTGGAAATCATATATGGCTGAGCCAATCAAAGC
CATAGGTTTTCTGGTCAATCCTCTAGAAAACCTTTAAAGACAACAAAACACATCATCAGT
CTCACAAAACCAATTAGACAAAAGCTTTTGTTTGGTTCAAAGAAAGAAGGTAACCTTCTG
CGAATACGGGAACTGAAATGTTTGACAAGATCTTCGATGGACATGTCGAGAAGCGCATCG
AGATCGACTCCTCTGAAAGAAAACTTTTTGAAGGTTCTCTTTTTCACAATTCCAGCCGCA
ACAACCTCTGGTTCCATATCCACAAAGATTGGAACTGACCATTGTTGGATCGATGAAACC
CTAAAAAATCCACAAAGCCTCAAAATAAAACAATGTATGCGCAAAAGACAAACAACTTAA
CTTAAGCACCAAATAAATAGGTAGCGCATCCTGTAACCCTAAAAATA

Last Updated on 06.12.2018 16:11