Sequences from confirmation amplicon


Confirmation sequences for line 470A06 and hit At5g59220 are shown.
Line ID 470A06
Locus At5g59220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23895380
Primers used for PCR 13an - 8474, show primer details
   
Sequence Name 39-K107000-0022-470-A06C-8409
GenBank Accession LN503370 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K107000-0022-470-A06C-8409
TGTAGATAGAGGATCTGATAAAGTTTATGAGTTTTAAGATTCTTTAATCCTTCGTGTCAC
TGTTATCAATGACTATATCATTCTCTCTCTCTCTCTTGTTTAAATTTGTTGTAACAGCCA
GACCGTCCGGACGAGCTAGACCGGATTCAAGCAGCGGGTGGTCGTGTTATCTACTGGGAT
GGCCCACGTGTCCTTGGAGTACTTGCAATGTCACGAGCCATTGGTCAGTTAATTGATTAC
ACTTGACGAAAACACTCTTGTCCCTATCCTAATGTTTGTACAAAGTTTTTCCGTACTTTC
AATAATGATGATTATTAACTCTCGTTTCGCCGCGGATTTTTTTTTTTTTTTCCGGGAAAA
ATTTCTTTGACCCCTTTTTTAATCCCAAAACCGGGGGAACCCGGGGCGGCCGGGCCCAAG
GAAAAAATTTTTTTTTTTCCCCAAGGGGAGGTTTTTGGGAACTTTTTTTAAAAAAAAACT
GATGGTGCCCCCTTTAAAAGGTTTTTAAAAAAAAAACTCAGGGGCCAATTTCCCCCCCCC
CA
   
Sequence Name 47-K107001-0022-470-A06C-13an
GenBank Accession LN506209 [GenBank]
Sequencing primer gene specific primer, 13an: 5'-GGTGATGATGATACTTGACCATTG-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K107001-0022-470-A06C-13an
TCGACGAGCTCATGCAGTTTCGTTTGAAACACGTCCCAAGACCGTCACTTGCGAGAATAA
GAAAATCGTCTCCGTTGGCCCGGTCCGTCACGGTTACCTCCGGTCTGCTGATTACATACG
GCTTCAAGTAATTATCTCCTGAAAAAAAAAAAAAAATCCCCGGGCAAACCAAGGTTAAAA
AACCTCCTTTTTGAAAAGTCCGAAAAACTTTTGAACAACCTTTGGAAAGGGGACAAAAGG
GTTTCCCCCAGGGGAAACCAATAAACGGCCAAGGGGCCCGGGACTTGGAAAGTCCCCCAG
GGACCCGGGGCCCCCCCCATAAAAAAACCCACCCCCCCCCGGTTTAAACCGGGTTAACCC
CCCCGGGAGGGTCGGGTTTTTCCACCAATTTTAACCAAAAAAGAAAAAAAAAAGGATTTT
TCCTTTGAAACCGGGGCCCCAAGGGATAAAAAATTTTAAAACCCCAAAACTTTTTTAAAA
CCCCCTTTCAAAGGGGTTTCTGGCCGGGAAAATCTCCGGGGACCCCCAAGGATTAGGGGG
GCCAATGGGGAAAAAAAAAAAAATTATTTCCCATTTTTTTTTCATTCAAAAAAGGAAAAT
CCCCCCCCCTTTTAAA

Last Updated on 06.12.2018 16:11