Genotyping and primer details


Genotyping and primer details for 470A06 - At5g59220
Line ID 470A06
Locus At5g59220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:23895380 of line 470A06 Chr5:23895380
   
Insertion allele amplicon 8474 - 13an
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 13an: 5'-GGTGATGATGATACTTGACCATTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:23894841
   
wt amplicon (over InsPos) 01bm - 13an
wt amplicon start Chr5:23894841
wt amplicon end Chr5:23895906
wt amplicon size 1066 bp
Locus specific primer 13an: 5'-GGTGATGATGATACTTGACCATTG-3'
2nd locus specific primer 01bm: 5'-AGCTAGTCCGTGAAGAGTTTGAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11