Sequences from confirmation amplicon


Confirmation sequences for line 470B08 and hit At5g37130 are shown.
Line ID 470B08
Locus At5g37130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:14685362
Primers used for PCR 24us - 8474, show primer details
   
Sequence Name 21-K117583-0022-470-B08C-24us
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 24us: 5'-CTGTGGTATGTTTGAACATTTTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K117583-0022-470-B08C-24us
TGATTTCCTTTACCGGTGCTGATCTTCCCTAGAAAATTTCGACCGTTTCCTGATTTATTT
TCACCATTAGCCGGAAA
   
Sequence Name 05-K117582-0022-470-B08C-8409
GenBank Accession LN488532 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K117582-0022-470-B08C-8409
ATTTAACATTCGCACCATCCATAGTATATGAAACATAAAGTAGGTGAGCAGTGAGACACC
AAGTTAAATAGTAAGGCATTCGTGAAGAAAACAATTAATTTTCAGCACCAACAGAGCAGA
CTAGTGTAACTGAAATGCTTACAGACTAGCAATGTTGTTCCAAGCCAGCCAATTGTCAGG
ATCAAGATGAACAGCTAAAGTGAATGCATCCAGTGCTTTCTGAAGATCCCTGGCCTATTG
TATCATGTCGGACATGTTAAATTAGTATATCTCTCAGCCAGCATGACAAGAATTCCAATT
GTTTATCAAGTTAGATTTGGAGTGGAAATATATTGCTACTAAGCTATAGGAGAAGATGAA
GTAAAATACCTTCAACGCAGCAGCACCAAGAGCAAACCAACCATCTGAATACAGAGAATT
TAACGCCATTGCAGCCTCCCTAGAGTAGGAGAAAAAAATGAGACATCTCACAAGTAAATC
AGCCTGAAGGGCCAAGTTCATAACTCCATTTCCCTTGTAGAGAAAGAAAAAATTAATAAG
GAAAGAACCAAAATGTT

Last Updated on 06.12.2018 16:11