Sequences from confirmation amplicon


Confirmation sequences for line 470E02 and hit At3g15605 are shown.
Line ID 470E02
Locus At3g15605 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5290819
Primers used for PCR 30aq - 8474, show primer details
   
Sequence Name 12-K106755-0022-470-E02C-8409
GenBank Accession LN491595 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K106755-0022-470-E02C-8409
GCCATTTACATTGAAATGGCCATGACAAGTGAAATGACAACTGAAAATAAGCGCAGAGTA
ATGCAACAACTTACCTTCTGAGCCGTCATCAGAGATCTATCCACCATGTTAAAATGGCCA
GTAAAGCTTCTACATCTTCCAATTTCGACTGGGACATTTTTGCTACCCGTTAGAGCTCTG
CAAATGGATCATCATATTAGATAGTAAAAGAAAGAAGGATTTGATGAAGTTAACATATAC
GTGAGAAGTAGAATATAAAAGAGTAGTAAGTAACCTCTGGCCAGATAACATCAGACAATC
TTCATTTAACCGCGACATAGCAGAATCAGCTGCCTTTGTGGTTCCAAAAATCACAAGAGC
CCTGCCTGTTTAGATAAATGCAGTTTAATTTGATATATTGACAGTTTTTTTTTTTTTCCC
AAACTTTAGGACCAAACAAATACCAAACGGGAGGGGGAAAAAATATCCTTACCCCCTTTT
GGGGCCAAAACTGGGAATTTAAGGGGATCTTTTAACCACCACCCCCTTTTTAAAAGGCTG
CCCAAAAAAAAACCGGACCAAAACAAAAGGGAACCGGCAAGAAAATTTATTTTTAACGGG
TTTTTAAAAAGGATGGCAATTCCCCCGTGTTTTTAACCAAAATTTGAAAAGGGCTTTCCT
TTGCCACCACCTTAAAAAAAAACCCAAAAATGGTTGAAAAATCCTAAACACTTCCCTTTC
CCCCCACCAATGTGTAAAA
   
Sequence Name 28-K106756-0022-470-E02C-30aq
GenBank Accession LN498864 [GenBank]
Sequencing primer gene specific primer, 30aq: 5'-ATTGAGAACCTGGAACCATCATAC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K106756-0022-470-E02C-30aq
TGATTCACAACATTCTCTGGATCTTATTTTATGTTGTTGTCAATGCAATGCCATCTACAT
ATGTTGATCAAAGACACGTGGAATTAGCAATCATTCTAAAAACCCTGTAAAATATAACTC
TCCTCGCCGTTTCCTTTTTGTTCTGTTCAGTTTCTTTTTAGGCAAGCATTCAAAGAAGGA
GTTGATGCTAAGATGATCCCTTCAAGTCCCATGTCTAGCCCACATAGTGGTATATGTATA
TTCTTACACCTTCCTGTTTGCTATTTGTTAGCTCATAATGTTAGGAAAAAAAAAAAAACC
GGCCAATATACAAAATAAAACGGCTTTTATCAAAACGGGCGGGGCTTTGGTATTTTTGGA
AACCAAAAGGGCACCTAATTCGGCTATGTCGGGGTAAAAAAAAAATGGTCGAATTTTATC
GGGCCAAAGGTAACTAACAACCCTTTAAAATCCAACTTCCCCCGAAAATTTAAATTTCAC
AAAACCCTTTTTTTTTTTACAACCAAAAAGAAGAACCCTTTGGCAAACCTCAAAGGGGAA
CAAAAAATGCCCCATTCAAATTGGAAAAATGAAAAACTTTTCCGGGCCTTTTAAACAGGG
GGAAAAAATCTCAAAAAACGGCCCAAAAGGAAATTTGTTGCATAACCCGGCCCTTATTTC
CATTTGTCATTTCCTTGGCCAGGGCCTTTTAATTTGAAAAGGGTTCCAGGCCCGGAAAAT
CTCCTGGGATCACCATGGATTAGAAGGGTCAATAGGGAAAAAAAAAAAAATAATTTCCCA
TTTTTTTTTCNTTTCAAAAAGGAAA

Last Updated on 06.12.2018 16:11