Sequences from confirmation amplicon


Confirmation sequences for line 470H07 and hit At3g61130 are shown.
Line ID 470H07
Locus At3g61130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22624852
Primers used for PCR HF58 - 8474, show primer details
   
Sequence Name 91-K101272-0022-470-H07C-8409
GenBank Accession LN515102 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>91-K101272-0022-470-H07C-8409
CGGACTGAGCATTTACATTGCTGAGGTTTATCCCAGCTGAACAAAATCCTCTTCCTGGAC
GATGACATCATTGTTCAGAAAGACTTGACTCCACTCTGGGAAGTTAACCTGAACGGCAAA
GTCAACGGTGCAGTCGAAACCTGTGGGGAAAGTTTCCACAGATTCGACAAGTATCTCAAC
TTTTCGAATCCTCACATTGCGAGGAACTTCAATCCAAATGCTTGTGGATGGGCTTATGGA
ATGAACATGTTCGACCTAAAGGAATGGAAGAAGAGAGACATCACTGGTATATACCACAAG
TGGCAAAACATGGTAAATAACTCTTTAATTCTTTGCAACAAATAGTCTAATGATGGTTTA
TTATTTTATTTATTGTGGTTTGCTCATTTTGGCTTGTGTTGTTTAGAATGAGAACAGGAC
ACTATGGAAGCTAGGGACATTGCCACCAGGATTAATAACATTCTACGGATTAACACATCC
CTTAAACAAGGCGTGGCATGTGCTGGGACTTGGATATAACCCGAGTATCGACAAGAAGGA
CATTGAGAATGCAGCAGTGGTTCACTATAACGGGAACATGAAACCATGGTTGGAGTTGGC
AATGTCCAAATATCGGCCGTATTGGACCAAGTACATCAAGTTTGATCACCCATATCTTCG
TCGTTGCAACCTTCATGAATAANATCAAATCTNTGTTAGATGATTTGAGGTAATTACTTC
TAATGTTCTGTTGTTCCCATTCCATTGCTCTTTATTCCACACATTGTTAATTGTTTATTC
ATATCGTTTTTTAGTCATTCTTTTCTTCTC
   
Sequence Name 91-K101278-0022-470-H07C-HF58
GenBank Accession LN515103 [GenBank]
Sequencing primer gene specific primer, HF58: 5'-TGTTTTTAACCCGAAAGAAGAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>91-K101278-0022-470-H07C-HF58
CGATTGAATAACATTAACAATGTGTGGAATAAAGAGCAATGGAATGGGAACAACAGAACA
TTAGAAGTAATTACCTCAAATCATCTAACAAAGATTTGATTTTATTCATGAAGGTTGCAA
CGACGAAGATATGGGTGATCAAACTTGATGTACTTGGTCCAATACGGCCGATATTTGGAC
ATTGCCAACTCCAACCATGGTTTCATGTTCCCGTTATAGTGAACCACTGCTGCATTCTCA
ATGTCCTTCTTGTCGATACTCGGGTTATATCCAAGTCCCAGCACATGCCACGCCTTGTTT
AAGGGATGTGTTAATCCGTAGAATGTTATTAATCCTGGTGGCAATGTCCCTAGCTTCCAT
AGTGTCCTGTTCTCATTCTAAACAACACAAGCCAAAATGAGCAAACCACAATAAATAAAA
TAATAAACCATCATTAGACTATTTGTTGCAAAGAATTAAAGAGTTATTTACCATGTTTTG
CCACTTGTGGTATATACCAGTGATGTCTCTCTTCTTCCATTCCTTTAGGTCGAACATGTT
CATTCCATAAGCCCATCCACAAGCATTTGGATTGAAGTTCCTCGCANTGTGAGGATTCGA
AAAGTTGAGATACTTGTCGAATCTGTGGAAACTTTCCCCACAGGTTTCGACTGCACCGTT
GACTTTGCCGTTCAGGTTAACTTCCCAGAGTGGAGTCNAGTCTTTCTGAACAATGATGTC
ATCGTCCAGGAAGAGGATTTTGTTCAGCTTGGGATAAACCTCAGCAATTGTAAATGGCTT
CATGT

Last Updated on 06.12.2018 16:11