Sequences from confirmation amplicon


Confirmation sequences for line 471A09 and hit At1g79870 are shown.
Line ID 471A09
Locus At1g79870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:30045092
Primers used for PCR 16ai - 8474, show primer details
   
Sequence Name 13-K106771-0022-471-A09C-8409
GenBank Accession LN492058 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K106771-0022-471-A09C-8409
TTACATTGAATAATCCATGTAGATTTCACCGAAGACGTCGCAGATCTCGCCATCGGGCTT
ATCCTGGCTCTCCTTCGACGGCTGTGTGAGTGCGATCGCTATGTAAGGAGCGGAAAATGG
AAGCAAGGTGAATTCCAACTCACTACCAAGGTTTCTACTTCTTACCCACTATTGATTGAT
TGGAAAAGAGCTCTCTGCCCTATCTTATCTTTGGTCTTCTATCTCTGCTTTAGCGTCACA
TTCTTTCTTTCTTTTGGTTGACTCAGTTTAGTGGAAAATCCGTGGGGATCATTGGTCTAG
GTAGAATTGGGACTGCCATCGCAAAGAGGGCTGAAGCCTTTAGCTGCCCAATCAATTACT
ACTCAAGAACCATTAAGCCTGATGTCGCCTACAAGTATTATCCGACGGTGG
   
Sequence Name 29-K106772-0022-471-A09C-16ai
GenBank Accession LN499308 [GenBank]
Sequencing primer gene specific primer, 16ai: 5'-ACCACCGTCGGATAATACTTGTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K106772-0022-471-A09C-16ai
TATTGATTGGGCAGCTAAGGCTTCAGCCCTCTTTGCGATGGCAGTCCCAATTCTACCTAG
ACCAATGATCCCCACGGATTTTCCACTAAACTGAGTCAACCAAAAGAAAGAAAGAATGTG
ACGCTAAAGCAGAGATAGAAGACCAAAGATAAGATAGGGCAGAGAGCTCTTTTCCAATCA
ATCAATAGTGGGTAAGAAGTAGAAACCTTGGTAGTGAGTTGGAATTCACCTTGCTTCCAT
TTTCCGCTCCTTACATAGCGATCGCACTCACACAGCCGTCGAAGGAGAGCCAGGATAAGC
CCGATGGCGAGATCTGCGACGTCTTCGGTGAAATCTACATGGATATATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGNTTAT
T

Last Updated on 06.12.2018 16:11