Sequences from confirmation amplicon


Confirmation sequences for line 472A12 and hit At1g12210 are shown.
Line ID 472A12
Locus At1g12210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4142438
Primers used for PCR 32sa - 8474, show primer details
   
Sequence Name 49-K116321-0022-472-A12C-32sa
GenBank Accession LN506954 [GenBank]
Sequencing primer gene specific primer, 32sa: 5'-GTTGTGGTCTACCACTAGCACTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K116321-0022-472-A12C-32sa
GAATGGCGTCATGCACTGAGGTTTTGACTTCTGCTACAGATTTTTCTGGCATGGAAGATG
AAATTCTTCCAATTTTAAAATATAGCTATGATAGCTTGAATGGGGAGGATGCGAAGTCGT
GTTTCCTATATTGCTCTCTATTTCCTGAAGATTTTGAAATCAGAAAAGAGATGCTTATTG
AATACTGGATTTGTGAGGGATTCATAAAAGAAAAGCAAGGTAGAGAAAAAGCATTCAACC
AAGGTTATGATATACTCGGTACTCTTGTCCGTTCATCTTTATTGTTGGAGGGAGCAAAGG
ATAAAGATGTCGTGAGTATGCATGATATGGTTCGTGAGATGGCTCTGTGGATATTTTCTG
ATCTTGGAAAGCATAAAGAGAGACTAAGAGAAAAAGAAAGAGTAAATACCAATTTTTTTC
AGCAATCTACATGCATGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCGGCTT
TTTT
   
Sequence Name 17-K116320-0022-472-A12C-8409
GenBank Accession LN494010 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K116320-0022-472-A12C-8409
CTGAAAAATTGGTATTTACTCTTTCTTTTTCTCTTAGTCTCTCTTTATGCTTTCCAAGAT
CAGAAAATATCCACAGAGCCATCTCACGAACCATATCATGCATACTCACGACATCTTTAT
CCTTTGCTCCCTCCAACAATAAAGATGAACGGACAAGAGTACCGAGTATATCATAACCTT
GGTTGAATGCTTTTTCTCTACCTTGCTTTTCTTTTATGAATCCCTCACAAATCCAGTATT
CAATAAGCATCTCTTTTCTGATTTCAAAATCTTCAGGAAATAGAGAGCAATATAGGAAAC
ACGACTTCGCATCCTCCCCATTCAAGCTATCATAGCTATATTTTAAAATTGGAAGAATTT
CATCTTCCATGCCAGAAAAATCTGTAGCAGAAGTCAAAACCTCAGTTGCATGACGCCATT
CTTGTATTGTCCTCTTGAATGACATGGTCTCACCAATGACATTCAGTGCTAGTGTAAACA
CCACAAC

Last Updated on 06.12.2018 16:11