Sequences from confirmation amplicon


Confirmation sequences for line 472C01 and hit At3g09600 are shown.
Line ID 472C01
Locus At3g09600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2947670
Primers used for PCR 54ns - 8474, show primer details
   
Sequence Name 62-K114626-0022-472-C01C-54ns
GenBank Accession LN510817 [GenBank]
Sequencing primer gene specific primer, 54ns: 5'-TAAAAGAAGTGCCTCCAACTCATT-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K114626-0022-472-C01C-54ns
AGTTGGTCATCACAAATCTTTTATCGCAAATTTAACACTTACAGTGGATTCTAAATCAGG
GTTTGATAAGTTAACTGTGAGGTTTCTCATCAATAACAGAACCTGTAAACACCATACTAT
GTGATTAGTGAAGAAATGCTAAGAGCTTGAACACCAATTAATCTGAAAGCTTGTAAACTC
TTGACTCACAGTTTCGAAATTTATAGGATCCATTTCCTTGAGCTTTTCCACATGGCCTCT
CGTTTCAGGATCAAAGACACTCCCAATGAAATTATAAACTTCAGCAAAATCAGGAACACC
TACAAAGATAGTATCAGTACAGTCTCCATCATGCATAAAAGTTAGTGAAAAAACATGGAA
TTACCAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 62-K114625-0022-472-C01C-8409
GenBank Accession LN510816 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K114625-0022-472-C01C-8409
ATGTTTTTTCACTAACTTTTATGCATGATGGAGACTGTACTGATACTATCTTTGTAGGTG
TTCCTGATTTTGCTGAAGTTTATAATTTCATTGGGAGTGTCTTTGATCCTGAAACGAGAG
GCCATGTGGAAAAGCTCAAGGAAATGGATCCTATAAATTTCGAAACTGTGAGTCAAGAGT
TTACAAGCTTTCAGATTAATTGGTGTTCAAGCTCTTAGCATTTCTTCACTAATCACATAG
TATGGTGTTTACAGGTTCTGTTATTGATGAGAAACCTCACAGTTAACTTATCAAACCCTG
ATTTAGAATCCACTGTAAGTGTTAAATTTGCGATAAAAGATTTGTGATGACCAACTTTCT
CAGAGTCTGAAACTATTTCACTCTCAACCAATGAGTTGGAGCATTTCTTTT

Last Updated on 06.12.2018 16:11