Sequences from confirmation amplicon


Confirmation sequences for line 472H11 and hit At5g23900 are shown.
Line ID 472H11
Locus At5g23900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8064595
Primers used for PCR 07vx - 8474, show primer details
   
Sequence Name 59-K118152-0022-472-H11C-07vx
GenBank Accession LN510101 [GenBank]
Sequencing primer gene specific primer, 07vx: 5'-GAATCTTATCGTAAGCCTTGAACG-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K118152-0022-472-H11C-07vx
CTCATGCTGCCTTGACCTGGCAATTGGCATGTAATCACCTTGAACTTGAGTAGCATTGGC
AAGCTCCTCTGGAGTAGAATCACCANGCCTGAAAACAAAAGATACAAAATTGTTATAACA
AGAACTCAAGAATCAAGAGGGTGTCTCAAATATACTACTCTAACTAAGATCTCAAATGAT
TGATACCTTGACTTGACGGGAACGGCGAGGGAACACCACCAACTTAGCCTTGTAAGTCTT
TAACCTTTGGACATTGGACTGAAGACCCTCCAAAGAACGGTTCTTTGAATATATTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAC
AGTTATT
   
Sequence Name 11-K118151-0022-472-H11C-8409
GenBank Accession LN491317 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K118151-0022-472-H11C-8409
ATTTCATTGAATATTTCAAGAACCGTTCTTTGGAGGGTCTTCAGTCCAATGTCCAAAGGT
TAAAGACTTACAAGGCTAAGTTGGTGGTGTTCCCTCGCCGTTCCCGTCAAGTCAAGGTAT
CAATCATTTGAGATCTTAGTTAGAGTAGTATATTTGAGACACCCTCTTGATTCTTGAGTT
CTTGTTATAACAATTTTGTATCTTTTGTTTTCAGGCTGGTGATTCTACTCCAGAGGAGCT
TGCCAATGCTACTCAAGTTCAAGGTGATTACATGCCAATTGCCAGTGTCAAGGCAGCTAT
GGAGCTAGTTAAGCTGACTGCAGATTTGAAGGCGTTCAAGGCTTACGATAAGATT

Last Updated on 06.12.2018 16:11