Sequences from confirmation amplicon


Confirmation sequences for line 473B05 and hit At1g57720 are shown.
Line ID 473B05
Locus At1g57720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:21380130
Primers used for PCR 48pm - 8474, show primer details
   
Sequence Name 42-K115290-0022-473-B05C-48pm
GenBank Accession LN504577 [GenBank]
Sequencing primer gene specific primer, 48pm: 5'-CATGGTCTCCTTTGTTACACTCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K115290-0022-473-B05C-48pm
GTAATACTCCTTTGGAAGATGCTTATTTGCGGGTCAGAGGGTCCTTTCAAGGTGAAGGGT
TTATGGCTTTTCCGTGGACCAGAGATCCCCAAGTTCATAATGGATGAGGTGTACGACATG
GAGCTGTACGAGTGGACTAAGGTTGATATCTCCGATGAAGCACAGAAGGAGCGTGTTAGC
CAGATGATTGAAGACGCTGAGCCATTTGAAGGCGAGGCTCTTTTGGACGCCAAATGCTTT
AAGTGAAAAGGCTCTTTGTATCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAANGGTTATT
   
Sequence Name 18-K115289-0022-473-B05C-8409
GenBank Accession LN494431 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K115289-0022-473-B05C-8409
CATTACATTGATACAAAGAGCCTTTTCACTTAAAGCATTTGGCGTCCAAAAGAGCCTCGC
CTTCAAATGGCTCAGCGTCTTCAATCATCTGGCTAACACGCTCCTTCTGTGCTTCATCGG
AGATATCAACCTTAGTCCACTCGTACAGCTCCATGTCGTACACCTCATCCATTATGAACT
TGGGGATCTCTGGTCCACGGAAAAGCCATAAACCCTTCACCTTGAAAGGACCCTCTGACC
CGCAAATAAGCATCTTTCCAAAGGAGTATTTACGTGCCAAGTCCATACGCTGAAGGAACC
CTCCTACCTTGTTGAGTGTAACAAAGGAGACCATG

Last Updated on 06.12.2018 16:11