Sequences from confirmation amplicon


Confirmation sequences for line 473B08 and hit At1g53660 are shown.
Line ID 473B08
Locus At1g53660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20034641
Primers used for PCR RU34 - 8474, show primer details
   
Sequence Name 29-K024845-022-473-B08C-8409
GenBank Accession LN499131 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K024845-022-473-B08C-8409
ATTTCCCGGACATGAAGCCATTTACAATTGTATACCTTAAAAACCTTCGTGAGGAGAAAA
CACAAAACAGAGGAAAAAGTCATGTGAAGTAATGTCAATCCAAGTGGATAAGGGAAGTTT
ATTTCCTTAGATGACAATACCCACTGCATCAGATTGAGTCAGAAATAAAAATTAGTGCTG
GAAAAAAGAAACTGAAAGAAGCTAAAGAGAAATGGAATGAACCAAAATTTACAAAAGTTT
GAACAGTAAAAAAGTTGTTTAAGCGAATAATCATTTGTGTAATGGAAGTACATAACTCAC
CATTGAACCATACATTTGAACCTACTGTTGTATTAACAAGAATTTTATTATTATTATATT
TCTTGTCCAAAACCAAATCGCATTGTAACAGAACTTTACCAAGATTGTCAAAGTTAAAAG
CTTTAAAATCTACTAATGTCCACCAGATCTAATAAAATCAAATCTTGAGCAAAAAGACTG
CAAATAGAAAACATAGATCCAATAACATATGAATGTGAGATTTGACTTAAAACCTTATTG
AAGAAGATTTGA
   
Sequence Name 30-K024845-022-473-B08C-RU34
GenBank Accession LN499568 [GenBank]
Sequencing primer gene specific primer, RU34: 5'-TCTCTCTAGCGGTCAAATCTTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K024845-022-473-B08C-RU34
TTTTAAGTCAAATCTCACATTCATATGTTATTGGATCTATGTTTTCTATTTGCAGTCTTT
TTGCTCAAGATTTGATTTTATTAGATCTGGTGGACATTAGTAGATTTTAAAGCTTTTAAC
TTTGACAATCTTGGTAAAGTTCTGTTACAATGCGATTTGGTTTTGGACAAGAAATATAAT
AATAATAAAATTCTTGTTAATACAACAGTAGGTTCAAATGTATGGTTCAATGGTGAGTTA
TGTACTTCCATTACACAAATGATTATTCGCTTAAACAACTTTTTTACTGTTCAAACTTTT
GTAAATTTTGGTTCATTCCATTTCTCTTTAGCTTCTTTCAGTTTCTTTTTTCCAGCACTA
ATTTTTATTTCTGACTCAATCTGATGCAGTGGGTATTGTCATCTAAGGAAATAAACTTCC
CTTATCCACTTGGATTGACATTACTTCACATGACTTTTTCCTCTGTTTTGTGTTTTCTCC
TCACGAAGGTTTTTAAGGTATACAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAA
ACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTC
GGAAATCTTTATTTCGACGTGTCTA

Last Updated on 06.12.2018 16:11