Sequences from confirmation amplicon


Confirmation sequences for line 473C11 and hit At5g43080 are shown.
Line ID 473C11
Locus At5g43080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17294597
Primers used for PCR KM53 - 8474, show primer details
   
Sequence Name 36-K032603-022-473-C11C-8409
GenBank Accession LN502115 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K032603-022-473-C11C-8409
GAAAATTTCTTTCTTAGAGCTATAAGAAGAAGAAATACAAGCAGCATAAGGTAACCCTAA
ATACTATTAAATGATAATTTGAAGAACAATGTTCTTGAGATTCACATGTTTCTTTTTCTA
TTTGGTTCAATACAGTTCAAGTGTGTAGCGACAATGCCTGTATCACCAGAGCTGCCTCTT
ACGGTTTTTGAAGATGTCAACATTTAGAGACAACTTTTGGTAATCGGCATAGATAGTATA
AAACCAGGTAGCGGTTTCGTTGGTTTCTTTCCTTGGTGTTTTGGCTTGTACATGTCTTAG
GACTACTAGTGTGATAAAGGATCTTAATATATGTTTAAAGATTTTGGTTACTTAATGATT
TTTTTTGTTTGGGTTATAATTCAAGCGTTCAACACCAATGTTGAACCATGCATTTCCATA
AATCTATCTAATGGGTTGATGCCTAAGCCAACCGATGCAACTATACGTAAAAGATTGAGA
TCTTGTGCACTACATATTCCATTCTTTGACATCCAAAGCTTTCCACTTATCTTCAGTTTT
CTTCGTTTTGATAAAAACAACGCTCCATCTTTGTTTTACATTATCGTCATCCAGACT
   
Sequence Name 36-K032603-022-473-C11C-KM53
GenBank Accession LN502116 [GenBank]
Sequencing primer gene specific primer, KM53: 5'-GTTCTAGTGTGGATGTCTGGATGA-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K032603-022-473-C11C-KM53
GACGTTGTTTTATCAAACGAAGAAACTGAAGATAAGTGGAAAGCTTTGGATGTCAAAGAA
TGGAATATGTAGTGCACAAGATCTCAATCTTTTACGTATAGTTGCATCGGTTGGCTTAGG
CATCAACCCATTAGATAGATTTATGGAAATGCATGGTTCAACATTGGTGTTGAACGCTTG
AATTATAACCCAAACAAAAAAAATCATTAAGTAACCAAAATCTTTAAACATATATTAAGA
TCCTTTATCACACTAGTAGTCCTAAGACATGTACAAGCCAAAACACCAAGGAAAGAAACC
AACGAAACCGCTACCTGGTTTTATACTATCTATGCCGATTACCAAAAGTTGTCTCTAAAT
GTTGACATCTTCAAAAACCGTAAGAGGCAGCTCTGGTGATACAGGCATTGTCGCTACACA
CTTGAACTGTATTGAACCAAATAGAAAAAGAAACATGTGAATCTCAAGAACATTGTTCTT
CAAATTATCATTTAATAGTATTTAGGGTTACCTTATGCTGCTTGTATTTCTCTCTTATAG
CTTCTAAAGAAAGAAATATATTCAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
AGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATT
TATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTA
CTT

Last Updated on 06.12.2018 16:11