Sequences from confirmation amplicon


Confirmation sequences for line 473E12 and hit At1g18580 are shown.
Line ID 473E12
Locus At1g18580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6396053
Primers used for PCR 22wj - 8474, show primer details
   
Sequence Name 81-K118585-0022-473-E12C-22wj
GenBank Accession LN514119 [GenBank]
Sequencing primer gene specific primer, 22wj: 5'-TAAACACATAAGCTTTGGCAAGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K118585-0022-473-E12C-22wj
TTGTGACCTCTTCCGTGAAGTTAAATGGGGAGGAGATACCATTTCGGTTCTCGTGTCTCT
CTCCTGTCAAAAGATGAGATTATCCAAAAGACACGAACATTAGCAGTTATAAACACTCAC
CCAATGTCTTATCTTTAGTAAATGAAATCACCAATTTCTACTACATCATCAAATTAAAAT
TCATTAGGTTATACAATTCACAATTTTTGTCAGCACAACAAGATCCAGATTTTTTCATGA
GGGAAGATCAAGCAGAGCAAAACTCAAATCTCAGCAATTAAATTTTATTAAATAAAGAGA
GAAAGACATACAAGTAAAAGCTGGGATGGATCTTGTTGATGGTGATAATGCTGAACGATG
AAGAGAACTAAACCAGCGACAGAGAAAGAACCAAGGAGAAACCATATCCAACTCGACAAT
CTCCTTCTACCTCGCCGCCGGTGATCCACCGGCCACCGCCTCATCTTCCGCTACAAGTTT
ACCCACCGTTTTCTGAATCGATGATAATAAACACTAAACCACGATTTCGAGCAGAACCTA
AAGAAATCGGAAATCTACCGACAGCGATCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCACGGGTTATTA
   
Sequence Name 51-K118584-0022-473-E12C-8409
GenBank Accession LN507644 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K118584-0022-473-E12C-8409
GATTTCCGATTTCTTTAGGTTCTGCTCGAAATCGTGGTTTAGTGTTTATTATCATCGATT
CAGAAAACGGTGGGTAAACTTGTAGCGGAAGATGAGGCGGTGGCCGGTGGATCACCGGCG
GCGAGGTAGAAGGAGATTGTCGAGTTGGATATGGTTTCTCCTTGGTTCTTTCTCTGTCGC
TGGTTTAGTTCTCTTCATCGTTCAGCATTATCACCATCAACAAGATCCATCCCAGCTTTT
ACTTGTATGTCTTTCTCTCTTTATTTAATAAAATTTAATTGCTGAGATTTGAGTTTTGCT
CTGCTTGATCTTCCCTCATGAAAAAATCTGGATCTTGTTGTGCTGACAAAAATTGTGAAT
TGTATAACCTAATGAATTTTAATTTGATGATGTAGTAGAAATTGGTGATTTCATTTACTA
AAGATAAGACATTGGGTGAGTGTTTATAACTGCTAATGTTCGTGTCTTTTGGATAATCTC
ATCTTTTGACAGGAGAGAGACACGAGAACCGAAATGGTATCTCCTCCCCATTTAAACTTC
ACGGAAGAGGTCACAAGTGCTTCCTCCTTCTCTAGGCAGTTAGCAGAGCAAATGACACTT
GCCAAAGCTATTGTGTTTA

Last Updated on 06.12.2018 16:11