Sequences from confirmation amplicon


Confirmation sequences for line 473G06 and hit At5g10370 are shown.
Line ID 473G06
Locus At5g10370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3263001
Primers used for PCR 16vc - 8474, show primer details
   
Sequence Name 59-K117671-0022-473-G06C-16vc
GenBank Accession LN510093 [GenBank]
Sequencing primer gene specific primer, 16vc: 5'-AAATTAACTTTCCAAGCTTTGGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K117671-0022-473-G06C-16vc
TATTCATGAACACCGNTTCTTCTCAACAACTGCACCCAACTGAACAAGATTTTGGACAGC
CATTGCAATGGCTTCCGGGACAGGCGCATCAACAAAGTTAAATTCTGCTATGTTGTTGAC
ACCCAGAGCAAGCATCCTCAAAAGTGCTACACCAAGATGGACCCTCCGAATTTCTGGTTC
CTGATTAAGATTCATTGAATCAAAATCGTTTTTTGAGTAGAGTCTGTAACATCTTCCGGG
TTCTGTCCTTCCAGCACGACCAGCACGTTGTCGAGCAGAACTCTGACTGACCCGGCACAC
CTTGAGGATGCTCATCCCCGTTCTAGGTTCATATTTACTCTCTTTCACCATCCCGGAATC
AATTACATACTTGACACCAGGAATAGTCAGCGACGTCTCTGCAATATTTGTGGCAAATAT
CACTTTCCGTCTCCCAGGATGGTTCTGGAAAACCCTAAACTGTTCTTCGAAGGAAAGCTT
TCCATGCAAAGGCAAAGCAATTGCAGACGGAGTTATAAACCTTTCGCATGCCCATTCTAC
CTCTGCTTGGGAAGTCAAGAATGCAAGGATGGTTCCTTCTTTCTCTGTTTTATGTATTTC
CACTGCCATTTTAACAACATCGCCAACATATGAAGCATACAATCTCCGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGC
   
Sequence Name 11-K117670-0022-473-G06C-8409
GenBank Accession LN491304 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K117670-0022-473-G06C-8409
AATGGCAGTGGAAATCATAAAACAGAGAAAGAAGGAACCATCCTTGCATTCTTGACTTCC
CAAGCAGAGGTAGAATGGGCATGCGAAAGGTTTATAACTCCGTCTGCAATTGCTTTGCCT
TTGCATGGAAAGCTTTCCTTCGAAGAACAGTTTAGGGTTTTCCAGAACCATCCTGGGAGA
CGGAAAGTGATATTTGCCACAAATATTGCAGAGACGTCGCTGACTATTCCTGGTGTCAAG
TATGTAATTGATTCCGGGATGGTGAAAGAGAGTAAATATGAACCTAGAACGGGGATGAGC
ATCCTCAAGGTGTGCCGGGTCAGTCAGAGTTCTGCTCGACAACGTGCTGGTCGTGCTGGA
AGGACAGAACCCGGAAGATGTTACAGACTCTACTCAAAAAACGATTTTGATTCAATGAAT
CTTAATCAGGAACCAGAAATTCGGAGGGTCCATCTTGGTGTAGCACTTTTGAGGATGCTT
GCTCTGGGTGTCAACAACATAGCAGAATTTAACTTTGTTGATGCGCCTGTCCCGGAAGCC
ATTGCAATGGCTGTCCAAAATCTTGTTCAGTTGGGTGCAGTTGTTGAGAAGAACGGTGTT
CATGAATTAACACAGGAAGGGCATTGTTTAGTCAAGCTGGGGCTGGAGCCAAAGCTTGGA
AAGTTAATTT

Last Updated on 06.12.2018 16:11