Sequences from confirmation amplicon


Confirmation sequences for line 474E05 and hit At1g65960 are shown.
Line ID 474E05
Locus At1g65960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24556829
Primers used for PCR NK19 - 8474, show primer details
   
Sequence Name 37-K102592-0022-474-E05C-8409
GenBank Accession LN502572 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K102592-0022-474-E05C-8409
AAGAAGGTATAGAGAAACAGAGCGTTTCAACATAGTCTCAAAGGACCAAGGAGTGCCAGT
CGTCGCCTTCTCTCTCAAGGACCATAGTTTCCACAACGAGTTCGAGATCTCTGAGATGCT
ACGTCGTTTTGGCTGGATCGTCCCAGCTTACACTATGCCTGCCGATGCACAGCACATCAC
GGTTCTGCGTGTTGTCATCAGGGAAGATTTCTCAAGAACACTCGCGGAGAGACTTGTTGC
TGATATTTCGAAGGTGCTTCATGAGCTAGATACCTTGCCTTCCAAGATATCTAAGAAGAT
GGGAATAGAAGGGATCGCGGAAAATGTAAAGGAGAAGAAGATGGAGAAGGAGATTCTGAT
GGAAGTTATTGTTGGATGGAGGAAGTTTGTGAAGGAGAGGAAGAAGATGATGTGGTTGTG
CTA
   
Sequence Name 37-K102593-0022-474-E05C-NK19
GenBank Accession LN502573 [GenBank]
Sequencing primer gene specific primer, NK19: 5'-TAGCACACACCATTCATCTTCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K102593-0022-474-E05C-NK19
CCACAATAACTTCATCAGAATCTCCTTCTCCTCTTCTTCTCCTTTACATTTTCCGCGATC
CCTTCTATTCCCATCTTCTTAGATATCTTGGAAGGCAAGGTATCTAGCTCATGAAGCACC
TTCGAAATATCAGCAACAAGTCTCTCCGCGAGTGTTCTTGAGAAATCTTCCCTGATGACA
ACACGCAGAACCGTGATGTGCTGTGCATCGGCAGGCATAGTGTAAGCTGGGACGATCCAG
CCAAAACGACGTAGCATCTCAGAGATCTCGAACTCGTTGTGGAAACTATGGTCCTTGAGA
GAGAAGGCGACGACTGGCACTCCTTGGTCCTTTGAGACTATGTTGAAACGCTCTGTTTTC
TCTATACCTTCTTTGAGAACCACCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGAT
CCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTC
GACGTGTCTACATTCACGTCCAAAT

Last Updated on 06.12.2018 16:11