Genotyping and primer details


Genotyping and primer details for 474E05 - At1g65960
Line ID 474E05
Locus At1g65960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:24556829 of line 474E05 Chr1:24556829
   
Insertion allele amplicon 8474 - NK19
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NK19: 5'-TAGCACACACCATTCATCTTCTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:24557252
   
wt amplicon (over InsPos) 05ks - NK19
wt amplicon start Chr1:24556347
wt amplicon end Chr1:24557252
wt amplicon size 906 bp
Locus specific primer NK19: 5'-TAGCACACACCATTCATCTTCTTC-3'
2nd locus specific primer 05ks: 5'-CTTCCTTTGGTTAAGAGCATCAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11