Sequences from confirmation amplicon


Confirmation sequences for line 476A12 and hit At5g63920 are shown.
Line ID 476A12
Locus At5g63920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25577254
Primers used for PCR JL32 - 8474, show primer details
   
Sequence Name 57-K032419-022-476-A12C-JL32
GenBank Accession LN509321 [GenBank]
Sequencing primer gene specific primer, JL32: 5'-CCAAGAAGTGAATGTGAATCAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K032419-022-476-A12C-JL32
GGGGAAAATGTAAGCCACTTAAATAAGCCCCATTATGTCTCAGGAAGAGTCCAGTAGATT
TAAACATATAAGACTGTCAGGATTTGACTAGAAAGCCAGCTCCCTGGGCACAGTTTTATG
CCAATAAACAAGCAGCCTCGTCCATACAGATAAAGCAAACTGTGTTTGGTTCTCCAAATT
TGTAGCAATTACTATTTGTTTGGGCCACTTTAATAATAATCATGACACCAGTTTTTGAAC
TAGAAACTACAATGCAAAGTTATGCCGTTCAATTGCATTATAGTTTAGTAGTTAAGTAAT
GTAACTTATAAGCAAGGACAAAGTAGTTTACCTCAAGGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACA
TTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATT
ATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACTTCA
   
Sequence Name 57-K032419-022-476-A12C-8409
GenBank Accession LN509320 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K032419-022-476-A12C-8409
TTACCTTGAGGTAACTACTTTGTCCTTGCTTATAAGTTACATTACTTAACTACTAAACTA
TAATGCAATTGAACGGCATAACTTTGCATTGTAGTTTCTAGTTCAAAAACTGGTGTCATG
ATTATTATTAAAGTGGCCCAAACAAATAGTAATTGCTACAAATTTGGAGAACCAAACACA
GTTTGCTTTATCTGTATGGACGAGGCTGCTTGTTTATTGGCATAAAACTGTGCCCAGGGA
GCTGGCTTTCTAGTCAAATCCTGACAGTCTTATATGTTTAAATCTACTGGACTCTTCCTG
AGACATAATGGGGCTTATTTAAGTGGCTTACATTTTTTCCCCAAACAGGAGCCTGTCTAA
CTGCTAAAATTTCTGATTCACATTCACCTTCTTGG
Primers used for PCR FQ46 - 3144, show primer details
   
Sequence Name 44-K033040-022-476-A12C-3144
GenBank Accession LN505085 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K033040-022-476-A12C-3144
CCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACC
CGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTAAACTATCAATATATCCTGCTGCCCTT
TTTCATACACGGGAATCACTGAACCACCCCATGATTCAAAGCGGTACACCTCAAGGTAAT
TTTTCTAGTCACACAAAGAAAATTAGGTCAGTATCAAATTATTTATTGAAGGGAAAGTAA
GCAATATATATGCTTGCAACAAAGAGCAGCTTTACATTTTATACTACCAAAACAGGAAAC
ATCTAATCAATCAAAGTAGTTTCACATTTTATTCCACAGAAATTACGACAAAAAAGTAGA
GAGTGAAGAGTCTTACGGCTAATATTGCTCTCCCAGATGCAGAAAAGCGCTCACCAGCGA
TATCAATCTCGACTGTAGTTTCAGCAGCAACTGCTGGCTGGGAAACACATGCTAGGTAAT
GGCGGACAACTAGCTCATATACATTCTGAAATAGTGGAAAAGAATGTTGGCTCCAACGTA
GAAACATATACCTATGCTCGGGTATGTTAAACCAAACTGACAGCCATACTTACAAGGTGA
TCTCTGCTCCAGTTAG
   
Sequence Name 12-K033040-022-476-A12C-FQ46
GenBank Accession LN491467 [GenBank]
Sequencing primer gene specific primer, FQ46: 5'-TTCTAGCGGAGAGTCTAACTGGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K033040-022-476-A12C-FQ46
GCTGTCAGTTTGGTTAACATACCCGAGCATAGGTATATGTTTCTACGTTGGAGCCAACAT
TCTTTTCCACTATTTCAGAATGTATATGAGCTAGTTGTCCGCCATTACCTAGCATGTGTT
TCCCAGCCAGCAGTTGCTGCTGAAACTACAGTCGAGATTGATATCGCTGGTGAGCGCTTT
TCTGCATCTGGGAGAGCAATATTAGCCGTAAGACTCTTCACTCTCTACTTTTTTGTCGTA
ATTTCTGTGGAATAAAATGTGAAACTACTTTGATTGATTAGATGTTTCCTGTTTTGGTAG
TATAAAATGTAAAGCTGCTCTTTGTTGCAAGCATATATATTGCTTACTTTCCCTTCAATA
AATAATTTGATACTGACCTAATTTTCTTTGTGTGACTAGAAAAATTACCTTGAGGTGTAC
CGCTTTGAATCATGGGGTGGTTCAGTGATTCCCGTGTATGAAAAAGGGCAGCAGGATATA
TTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTC
CAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGC
GTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGG
AACGTCTTCTTTTTCCACGATGCTCCTCGTG

Last Updated on 06.12.2018 16:11