Genotyping and primer details


Genotyping and primer details for 476A12 - At5g63920
Line ID 476A12
Locus At5g63920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:25577254 of line 476A12 Chr5:25577254
   
Insertion allele amplicon 3144 - FQ46
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer FQ46: 5'-TTCTAGCGGAGAGTCTAACTGGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:25576733
   
Insertion allele amplicon 8474 - JL32
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JL32: 5'-CCAAGAAGTGAATGTGAATCAGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:25577675
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11