Sequences from confirmation amplicon


Confirmation sequences for line 476C04 and hit At5g65400 are shown.
Line ID 476C04
Locus At5g65400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26132081
Primers used for PCR 41uw - 8474, show primer details
   
Sequence Name 22-K117753-0022-476-C04C-41uw
GenBank Accession LN496293 [GenBank]
Sequencing primer gene specific primer, 41uw: 5'-AATTTTATGCCTTCATGGATTCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K117753-0022-476-C04C-41uw
CGGTAAGGTAATGGCCGGACACAATTCTCCGAGATCTCGACCTCGATTTCCTTGACGCTC
CTTTTCCGGCCACCGGGAAATCGGACGTCGAGCGTTTCTTTGACCCACCGTATTACGAGT
GGTACCAGGCTAACAAGGTTCGTTGCAACAAAATCCTAAATTTCCTTGAATTCAGTATTG
ATCATTGACACTGTTAACACTATCAGAGTAAAGGTTGTTTTTTTTCTCTAGAAAACAGAG
AGATTTGGTCTAAGTTTGTATACATAATTTGATTTGTGTGTGTGTGTGTTTGCAGGGGTT
TAAGGAATATAGGAATTTTGAAGAGTGTTTGGCTTATATAGAGGACTACATGATAAAGAA
TGGACCTTTTGATGGTCTTCTTGGTTTTTCTCAGGTTTGTTTTGATTCTTTGGCGAGATT
TTAGTACTGATCACTTTCATATATATCAGCAGGAGGATCGTGGACCGTTGATTCTTGATC
CATTCTTGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCC
   
Sequence Name 06-K117752-0022-476-C04C-8409
GenBank Accession LN488992 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K117752-0022-476-C04C-8409
TACATTGAAATATCCTGCAAGAATGGATCAAGAATCAACGGTCCACGATCCTCCTGCTGA
TATATATGAAAGTGATCAGTACTAAAATCTCGCCAAAGAATCAAAACAAACCTGAGAAAA
ACCAAGAAGACCATCAAAAGGTCCATTCTTTATCATGTAGTCCTCTATATAAGCCAAACA
CTCTTCAAAATTCCTATATTCCTTAAACCCCTGCAAACACACACACACACAAATCAAATT
ATGTATACAAACTTAGACCAAATCTCTCTGTTTTCTAGAGAAAAAAAACAACCTTTACTC
TGATAGTGTTAACAGTGTCAATGATCAATACTGAATTCAAGGAAATTTAGGATTTTGTTG
CAACGAACCTTGTTAGCCTGGTACCACTCGTAATACGGTGGGTCAAAGAAACGCTCGACG
TCCGATTTCCCGGTGGCCGGAAAAGGAGCGTCAAGGAAATCGAGGTCGAGATCTCGGAGA
ATTGTGTCCGGCCATTTACCTATACCGGCTTGGAGAATCCGACCACTAGTTCTGAATCCA
T

Last Updated on 06.12.2018 16:11